摘要
目的建立同时测定红参、麦冬及其制剂参麦注射液中果糖、葡萄糖、蔗糖、麦芽糖的含量。方法采用Alltech Prevail Carbohydrate ES（4.6 mm×250 mm,5μm）色谱柱,流动相为乙腈-水溶液（72∶28）,流速：1.0 m L·min^-1,柱温：25℃,ELSD氮气压力：3.5 bar,漂移管温度40℃。结果在确定的分析条件下,果糖、葡萄糖、蔗糖、麦芽糖分别在1.01-20.20μg,0.48-9.66μg,0.95-19.08μg,0.96-19.28μg内呈良好的对数线性关系,回收率均在95%-105%内。结论该方法简便、准确、稳定,适合用于红参、麦冬及其制剂参麦注射液中各糖的含量测定。
OBJECTIVE To establish a method of simultaneous determining for fructose, glucose, sucrose and maltose in red ginseng, Ophiopogon japonicus and Shengmai injection. METHODS Alltech Prevail Carbohydrate ES（4.6 mm×250 mm, 5 μm） was adopted with acetonitrile-water（72∶28） as molile phase. The flow rate was 1.0 m L·min^-1. The column temparature was maintained at 25 ℃. ELSD was was adopted with the drift temperature of 40 ℃. And Nitrogen pressure was 3.5 bar. RESULTS The linear range of fructose, glucose, sucrose and maltose were 1.01-20.20 μg, 0.48-9.66 μg, 0.95-19.08 μg and 0.96-19.28 μg, respectively. The corresponding average recovery rates were 95%-105%. CONCLUSION The method was simple, accurate and reproducible and can be used for the quality control of Red ginseng, Ophiopogon japonicus and its preparation of Shengmai Injection.
出处
《中国现代应用药学》
CAS
CSCD
2014年第11期1395-1399,共5页
Chinese Journal of Modern Applied Pharmacy
