摘要
目的:探讨bcl-2、bax在胸部撞击致大鼠急性肺损伤(ALI)中的表达改变及其意义。方法:健康雄性SD大鼠24只,随机分为正常对照组和创伤后3h,12h,24h组。采用TUNEL法以及免疫组化技术观察肺组织bcl-2、bax表达及细胞凋亡的变化,观察病理形态。结果:与对照组比较,各创伤组肺组织内细胞凋亡指数增加,并随着病程延长逐渐上升(P<0.05);bax基因表达明显增强,并随着病程延长而逐渐增高(P<0.01);bcl-2基因表达亦明显升高,但随着病程延长呈降低趋势(P<0.01);bcl-2/bax比值降低,该比值也有随着病程延长而逐渐下降的趋势(P<0.01)。各创伤组光镜下见肺组织损伤明显。结论:肺组织细胞凋亡及bcl-2、bax基因表达参与胸部撞击致ALI时的肺损伤,并在其发病机制中起着一定的作用。
Objective：To evaluate the effects of apoptosis and study the expression ofapoptosis related genes hcl-2 and bax in acute lung injury following blunt chest trauma in rats.Methods：An animal model of acute lung injury was established in SD rat by impacting chest.24 SD rats were divided equally and randomly into normal group （n=6）.ALI model group （include 3h group,12h group,24h group;n=6 eaeh）.The pathological changes and cell apoptosis were observed with TUNEL,immunochemieal methods.Results：As compared with M group,in every group after chest trauma.apoptotic index markedly increased,and increased gradually with the course advanced （P〈0.05）;expressions of bax and bcl-2 also markedly increased,and expressions of bax increased gradually with the course advanced,but expressions of bcl-2 was reduced gradually with the disease prolonged （P〈0.01）;the ratio of bcl-2/bax markedly decreased, and reduced gradually with the disease prolonged （P〈0.01）;lung tissue injuries were significant under a lightmicroscope.Conelusion：The apoptosis and expression of bax and bcl-2 in lung tissue might be involved in pathogenesis of the lung injury lbllowing blunt chest trauma
出处
《中国医药导刊》
2010年第3期467-468,473,共3页
Chinese Journal of Medicinal Guide
