期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

黄体酮促进培养乳腺癌细胞的增殖和迁移 被引量：2

Progesterone promotes the proliferation and migration of cultured breast cancer cells
暂未订购
导出
摘要 目的研究黄体酮对乳腺癌细胞增殖和迁移的影响。方法100nM黄体酮刺激黄体酮受体阳性的乳腺癌细胞株T-47D和MCF-748小时后,用MTT方法检测细胞增殖,用细胞划痕愈合实验检测细胞迁移,用Western blotting检测细胞内E-Cadherin的表达。结果黄体酮刺激MCF-7和T-47D细胞的增殖和迁移;并且可以下调MCF-7和T-47DE-Cadherin的表达。结论黄体酮能够促进培养乳腺癌细胞的增殖和迁移,提示抗黄体酮治疗可能有临床意义。 Objective To investigate the effects of progesterone on the growth and migration of breast cancer cells. Methods MCF-7 and T-47D cells were cultured in DMEM and stimulated with 100 nmol/L progesterone for 48 h, and the cell proliferation was evaluated by MTT assay, cell migration by wound-healing assay and E-catherin expression by Western blotting. Results Progesterone stimulated the cell proliferation and migration and down-regulated the expression of E-catherin in both MCF-7 and T-47D cells. Conclusions Progesterone stimulates the cell proliferation and migration of cultured breast cancer cells, suggesting the clinical significance of anti-progesterone therapy in breast cancer.
作者 赵嘉佳 王辛 霍中军 罗深秋 熊静波
机构地区 南方医科大学细胞生物学教研室 南方医科大学病理学教研室
出处 《南方医科大学学报》 CAS CSCD 北大核心 2010年第3期443-446,共4页 Journal of Southern Medical University
基金 国家自然科学基金(30470673) 广东省自然科学基金(4020364) 教育部留学回国人员启动基金(教外司留[2004]176号)
关键词 黄体酮 乳腺癌 细胞增殖 细胞迁移 progesterone breast cancer cell proliferation cell migration
分类号 R737.9 [医药卫生—肿瘤]
  • 相关文献

参考文献28

  • 1Graham JD, Clarke CL. Physiological action of progesterone in target tissues [ J ]. Endocr Rev, 1997, 18(4): 502-19.
  • 2Lydon JP, DeMayo F J, Funk CR, et al. Mice lacking progesterone receptor exhibit pleiotropic reproductive abnormalities [J ]. Genes Dev, 1995, 9(18): 2266-78.
  • 3Rossouw JE, Anderson GL, Prentice RL, et al. Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results From the Women's Health Initiative randomized controlled trial[J]. JAMA, 2002, 288(3): 321-33.
  • 4Lydon JP, Ge G, Kittrell FS, et al. Murine mammary gland carcinogenesis is critically dependent on progesterone receptor fimction [ J ]. Cancer Res, 1999, 59(17): 4276-84.
  • 5Sartorius CA, Harvell DM, Shen T, et al. Progestins initiate a luminal to myoepithelial switch in estrogen-dependent human breast tumors without altering growth[J].Cancer Res, 2005, 65(21): 9779-88.
  • 6Moore MR, Conover JL, Franks KM. Progestin effects on long-term growth, death, and Bcl-xL in breast cancer cells [J]. Biochem Biophys Res Commun, 2000, 277(3): 650-4.
  • 7Liang Y, Hyder SM. Proliferation of endothelial and tumor epithelial cells by progestin-induced vascular endothelial growth factor from human breast cancer cells: paracrine and autocrine effects [Jl. Endocrinology, 2005, 146(8): 3632-41.
  • 8Liang Y, Besch-Williford C, Brekken RA, et al. Progestin-dependent progression of human breast tumor xenogratts: a novel model for evaluating antitumor therapeutics [J]. Cancer Res, 2007, 67 (20): 9929-36.
  • 9Alkhalaf M, ElMowafy A, Karam S. Growth inhibition of MCF-7 human breast cancer cells by progesterone is associated with cell differentiation and phosphorylation of Akt protein [ J ]. Eur J Cancer Prev, 2002, 11(5): 481-8.
  • 10Caldon CE, Lee CS, Sutherland RL, et al. Wilms' tumor protein 1: an early target of progestin regulation in T-47D breast cancer cells that modulates proliferation and differentiation [ J ]. Oncogene, 2008, 27(1): 126-38.

同被引文献35

  • 1Ford D, Easton DF, Stratton M, et al. Genetic heterogeneity and penetrance analysis of the BRCAI and BRCA2 genes in breast cancer families[J]. Am J Hum Genet, 1998, 62(3): 676-89.
  • 2Thompson ME, Jensen RA, Obermiller PS, et al. Decreased expression of BRCAI accelerates growth and is often present during sporadic breast cancer progression [J]. Nat Genet, 1995, 9 (4): 444-50.
  • 3Dobrovic A, Simpfendorfer D. Methylation of the BRCA1 gene in sporadic breast cancer[J]. Cancer Res, 1997, 57: 3347-50.
  • 4Mullah PB, Quinn JE, Harkin DE The role of BRCA1 in transcriptional regulation and cell cycle control [J]. Oncogene, 2006, 25(43): 5854-63.
  • 5Fan S, Wang J, Yuan R, et al. BRCA1 inhibition of estrogen receptor signaling in transfected cells[J]. Science, 1999, 284(5418): 1354-6.
  • 6Maor SB, Abramovitch S, Erdos MR, et al. BRCA1 suppresses insulin-like growth factor-I receptor promoter activity: potential interaction between BRCA1 and Spl [J]. Mol Genet Metab, 2000, 69(2): 130-6.
  • 7Burga LN, Hu H, Juvekar A, et al. Loss of BRCA1 leads to an increase in epidermal growth factor receptor expression in mammary epithelial cells, and epidermal growth factor receptor inhibition prevents estrogen receptor-negative cancers in BRCAl-mutant mice[J]. Breast Cancer Res, 2011, 13(2): R30.
  • 8Aprelikova ON, Fang BS, Meissner EG, et al. BRCAl-associated growth arrest is RB-dependent[J]. Proc Natl Acad Sci USA 1999, 96 (21): 11866-71.
  • 9Williamson EA, Dadmanesh F, Koeffler HP. BRCA1 transactivates the cyclin-dependent kinase inhibitor p27(Kipl) [J]. Oncogene, 2002, 21 (2): 3199-206.
  • 10Seery LT, Knowlden JM, Gee JM, et al. BRCA1 expression levels predict distant metastasis of sporadic breast cancers[J]. Int J Cancer, 1999, 84(5): 258-62.

引证文献2

二级引证文献12

南方医科大学学报
2010年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部