摘要
目的观察乏氧射线双调控的TK腺病毒载体-Ad.HRE.CArG.HSV-TK联合放疗对乳腺癌细胞Bcap37裸鼠移植瘤生长的影响。方法皮下接种细胞于BALB/C(nu/nu)裸鼠建立移植瘤模型。当肿瘤直径达8～10mm时,将裸鼠分为对照组(control)、载体组(Ad)、照射组(RT)和载体合并照射组(Ad+RT)。治疗开始为第1天,于第1、5天瘤内多点注射0.2ml表达载体,给药次日起腹腔注射GCV(40mg/kg),每日1次,连续14天。第2、4、6天给予2GyX线照射。每3天测量肿瘤长短径,计算肿瘤体积。治疗后30天处死裸鼠,剥离瘤块称重,计算抑瘤率。对肿瘤组织行HE染色和AnnexinV法检测凋亡。结果实验组平均肿瘤体积和瘤重均显著小于对照组(P<0.05)。HE染色可见肿瘤细胞变性坏死和凋亡细胞,AnnexinV检测显示实验组凋亡率明显高于对照组(P<0.05)。结论腺病毒载体联合放疗对Bcap37裸鼠移植瘤生长具显著抑制作用并可介导凋亡,为乳腺癌进一步的放射基因治疗研究奠定了基础。
Objective To study the effects of TK adenovirus vecto-Ad. HRE. CArG. HSV-TK regulated by hypoxia and radiation combined with radiotherapy on the growth of transplanted tumors of breast cancer Bcap37 in nude mice. Methods The models of transplanted tumors in BALB/C（nu/nu） nude mice were established by subcutaneous inoculation ceils. When tumor diameter increased to 8- 10mm, the mice were allocated to be control group, vector group（Ad）, radiation group（RT） and vector plus radiation group（Ad＋ RT）. Day 1 was defined as treatment outset. On day 1 and day 5,0. 2 ml adenovirus vector was offered by tumor multi-point injection. The next day of injection, GCV（40 mg/kg） was given using intraperitoneal injection,once on everyday for 14 days. X-ray irradiation of 2Gy was rendered on day 2, day 4 and day 6. The size of the tumor was measured every 3 days,and the volume of the tumor was cal culated. After 30 days,the mice were killed,and the tumor was weighed and inhibition rate was calculated. HE staining and Annexin V detection were proceded to detect the apotosis of cancer cells in tumor tis sues. Results The average volume and weight in experimental group were smaller than that in the control group（P〈0. 05）. Necrosis and apoptosis were observed from the HE staining tissue. The rate of apopto sis in experimental group was significantly higher than that in the control group（P〈0. 05）. Conclusion Adenovirus vector combined with radiotherapy had significant inhibition to the Bcap37 transplanted tumor in nude mice and induced cell. This provided the foundation for further gene radiation therapy of breast cancer.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第3期259-262,共4页
Cancer Research on Prevention and Treatment
基金
山东省卫生厅科研基金资助项目(2005HZ087)
