摘要
目的观察硒对慢性氟中毒鸡血液、脾脏CD4^＋、CD8^＋淋巴细胞亚群以及脾淋巴细胞凋亡的影响，并探讨其作用机制。方法将8日龄海蓝褐雏鸡180只按体质量随机分为3组：对照组、氟中毒组、硒拮抗组，每组60只，分别饲以含氟195、1000、1000mg／kg、含硒0.08、0．08、4．00mg／kg的全价日粮。于30、60、90d用流式细胞术检测外周血和脾脏中CD4^＋、CD8^＋淋巴细胞水平，TUNEL法检测脾淋巴细胞凋亡情况。结果与对照组比较，30、60、90d氟中毒组外周血CD4^＋淋巴细胞水平降低[（35．36±4．27）％vs（24．29±2．96）％、（47．65±5．42）％vs（41．62±3．96）％、（49．58±3．98）％vs（42．35±6．03）％，P〈0．05或〈0．01]，CD4^＋／CD8^＋比值也明显降低[（1．701±0．145）％vs（1．393±0．163）％、（2．712±0．345）％vs（1．781±0．201）％、（2．438±0．356）％vs（1．973±0．229）％，P〈0．05或〈0．01]；与氟中毒组比较，30、60、90d硒拮抗组外周血中CD4^＋淋巴细胞水平升高[（29．40±3．38）％、（45．40±6．01）％、（46．85±5．25）％，P〈0．05或〈0．01]，60、90d时CD4^＋／CD8^＋比值明显回升[（2．004±0．314）％、（2．211±0．229）％，P均〈0．01]。与对照组比较，30、60、90d氟中毒组脾脏中CD4^＋淋巴细胞水平降低[（47．33±5．35）％vs（41．91±4．83）％、（49．28±5．24）％vs（41．26±4．56）％、（34．31±4．15）％vs（29．33±2．89）％，P均〈0．01]，CD4^＋／CD8^＋比值也明显降低[（1．927±0．244）％vs（1．525±0．265）％、（1．847±0．224）％vs（1．640±0．198）％、（1．265±0．174）％vs（0．878±0．092）％，P〈0．05或〈0．01]；与氟中毒组比较，60、90d硒拮抗组脾脏中CD4^＋淋巴细胞水平升高[（44．87±5，43）％、（32．62±3．37）％，P均〈0．05]，而30、60、90d时CD4^＋／CD8^＋比值明显回升[（1．703±0．201）％、（1．772±0．215）％、（0．991±0．124）％，P〈0．05或〈0．01]。30、60、90d时氟中毒组鸡脾淋巴细胞凋亡指数[（2．31±0．36）％、（2．76±0．22）％、（3．04±0．29）％]明显高于对照组[（1．14±0．21）％、（1．23±0．23）％、（1．29±0．20）％]，而60、90d时硒拮抗组[（2．42±0．32）％、（2．73±0．39）％]低于氟中毒组，差异均有统计学意义（P〈0．05或〈0．01）。结论一定程度的硒摄人能减少氟中毒鸡淋巴细胞的凋亡、改善失衡的淋巴细胞亚群来拮抗氟的毒性。
Objective To study the effect of selenium on peripheral and splentic T-cell subset, apoptosis of spleen cells in fluorosis chicken and its mechanism. Methods One hundred and eighty 8-day Hailanhe chicks were randomly divided into 3 groups （each 60） ： ①control group： 195 mg/kg fluoride and 0.08 mg/kg of selenium; ②fluorine group： 1000 mg/kg fluoride and 0.08 mg/kg of selenium;③selenium antagonism group： 1000 mg/kg fluoride and 4.00 mg/kg of selenium. On 30^th, 60^th, 90^th day, peripheral and splentic CD4^＋, CD8^＋ T-cell subset analyses underwent flow cytometry and apoptosis of spleen cells were detected by TUNEL for study subjects. Results Compared with control group, the CD4^＋ T-cell subset of peripheral in fluorine group was decreased obviously in 30,60,90 days[ （35.36 ± 4.27）% vs （24.29 ± 2.96）%, （47.65 ± 5.42）% vs （41.62 ± 3.96）%, （49.58 ± 3.98）% vs （42.35 ± 6.03）%, P〈 0.05 or 〈 0.01 ], CD4^＋/CD8^＋ ratio also was decreased obviously[ （1.701 ± 0.145）% vs （1.393 ± 0.163）%,（2.712 ± 0.345）% vs （1.781 ± 0.201）%, （2.438 ± 0.356）% vs （1.973 ± 0.229）%, P〈 0.05 or 〈 0.01 ]. Compared with fluorine group, the CD4^＋ T-cell subset of peripheral in selenium antagonism group [ （29.40 ± 3.38）%, （45.40 ± 6.01）%, （46.85 ± 5.25）%, P 〈 0.05 or 〈 0.01 ] was increased obviously in 30,60,90days, CD4^＋/CD8^＋ ratio in 60,90 days[ （2.004 ± 0.314）%, （2.211 ± 0.229）%, all P 〈 0.01] also was increased obviously. Compared with control group, the CD4^＋ T-cell subset of spleen cells in fluorine group was decreased obviously in 30,60,90 days[（47.33 ± 5.35）% vs （41.91 ± 4.83）%, （49.28 ± 5.24）% vs （41.26 ± 4.56）%, （34.31 ± 4.15）% vs （29.33 ± 2.89）%, all P 〈 0.01 ], CD4^＋/CD8^＋ ratio also was decreased obviously[ （1.927 ± 0.244）% vs （1.525 ± 0.265）%, （1.847 ± 0.224）% vs （1.640 ± 0.198）%, （1.265±0.174）% vs （0.878 ± 0.092）%, P 〈 0.05 or 〈 0.01 ]. Compared with fluorine group, the CD4^＋ T-cell subset of spleen ceils in selenium antagonism group in 60,90 days [ （44.87 ± 5.43 ）%, （32.62 ± 3.37 ）%, all P 〈 0.05 ] was increased obviously, CD4^＋/CD8^＋ ratio in 30,60, 90 days[ （1.703 ± 0.201 ）%, （1.772 ± 0.215）%, （0.991 ±0.124）%, P 〈 0.05 or 〈 0.01 ] also was increased obviously. The apoptosis ratio of spleen cells in fluorine group in 30,60,90 days[ （2.31 ± 0.36）%, （2.76 ± 0.22）%, （3.04 ±0.29）% ] was higher than that in control group [ （1.14 ± 0.21 ）%, （1.23±0.23）%, （1.29 ±0.20）%, P 〈 0.01 ]. The apoptosis ratio of spleen cells in selenium antagonism group in 60,90 days[ （2.42 ± 0.32）%, （2.73 ± 0.39）%] was lower than that in fluorine group（P 〈 0.05 or 〈 0.01 ）. Conclusion A certain concentration of selenium can antagonize the immunity inhibition of fluorine by decreasing apoptosis and improving the unbalance of T-cell subset.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2010年第2期142-145,共4页
Chinese Jouranl of Endemiology
关键词
硒
氟
细胞凋亡
免疫
T淋巴细胞亚群
Selenium
Fluorine
Apoptosis
Immunity
T-lymphocyte subsets
