摘要
机体的免疫应答是一个复杂的生物学过程,它以体液和细胞免疫为主,二者相互影响,相互作用,而构成了一个动态整体。本研究通过FACS技术检测SPF鸡接种共表达IBVS1基因和鸡γ-干扰素基因的重组鸡痘病毒疫苗后外周血中CD4+、CD8+和TCRδγ+三种亚类T淋巴细胞的数量的变化,初步探讨了干扰素对鸡外周血中T淋巴细胞亚类动态分布的影响。结果表明,含有干扰素基因的重组鸡痘病毒能显著增高机体特异性的CD8+和TCRδγ+T淋巴细胞水平,CD4+T淋巴细胞比例则要低于其他的疫苗免疫组。
The immune response is a complicated process, it contains humoral immunity and cellular immunity, they affect each other and forms a dynamic system. In this study, FACS technique was used to detect the quantitative changes of CD4^＋, CD8^＋ and TCRγ^＋ T lymphocyte in SPF chickens peripheral blood after inoculating recombinant fowlpox virus which co-expressing IBV S1 gene and ChIFNγ gene, in order to discuss the interferon＇s affection on T lymphocyte dynamic distribution in peripheral blood. The results showed that the recombinant fowlpox virus can boost the maturation of CD8^＋ and TCRδγ^＋ T lymphocyte, while decrease the ratio of CD4~T lymphocyte compared to other groups.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2006年第5期539-543,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
国家"863"计划资助项目(2003AA213020)
