摘要
白羊草幼穗在附加有２，４－Ｄ１．０ｍｇ／Ｌ、水解酪蛋白５００或１０００ｍｇ／Ｌ的Ｎ６或ＭＳ培养基中，在２５±１℃，暗光培养条件下，两周时形成０．５ｃｍ２大小的愈伤组织块。愈伤组织的发生率为８４％。培养３周后形成旺盛生长的愈伤组织。其分化是在含有ＮＡＡ０．５ｍｇ／Ｌ、ＫＴ１．０ｍｇ／Ｌ的ＭＳ培养基上或者在附加有ＫＴ１．０ｍｇ／Ｌ、ＩＡＡ０．４ｍｇ／Ｌ、ＢＡ１．０ｍｇ／Ｌ的Ｎ６培养基上２～４周后均可得到旺盛生长的分化植株，将该植株移至不含激素的Ｎ６培养基内，在光照下进行培养，形成绿色分化植株。３～４周后，将分化植株移入土壤中，植株生长良好。
Cultured in darkness and under the temperature condition of 25±1℃ for 2 weeks, the young spikelet of Bothriochloa ischaemum, laid on N 6 or MS medium containing 2,4 D (1.0 mg/L) and CH (500 or 1 000 mg/L), produced callus of 0.5 cm 2 in size and with a rate of occurrence as high as 84%. The callus became well grown in one week and then it was cultured into vigorous shoots in 2～4 more weeks on a new medium of MS+NAA (0.5 mg/L)+KT (1.0 mg/L) or N 6+KT (1.0 mg/L)+IAA (0 4 mg/L)+BA (1.0 mg/L). The formed shoots were then transferred onto a medium of N 6 without hormones, and, under light, they developed into green plants in 3～4 weeks. The plants were successfully transplanted into soil and grew well.
出处
《草业科学》
CAS
CSCD
北大核心
1998年第6期19-20,共2页
Pratacultural Science
关键词
白羊草
组织培养
幼穗
Bothriochloa ischaemum, tissue culture
