摘要
目的：检测急性白血病细胞内ｐ５３基因表达情况。方法：３Ｈ－ｄＡＴＰ标记基因探针的斑点杂交。结果：急性淋巴细胞白血病（ＡＬＬ）表达ｐ５３ＲＮＡ水平与正常对照类似，急性非淋巴细胞白血病（ＡＮＬＬ）中５９．４％表达低水平ｐ５３或表达缺失，ｐ５３表达缺失在Ｍ４、Ｍ５中多见。结论：急性白血病细胞中存在ｐ５３ＲＮＡ表达异常。
Objective: p53 gene expression was detected in acute Leukemia cells.Methods: DNA RNA Dot blot with 3H dATP probe.Results: p53 mRNA in all cells was normal,p53 RNA expression was very lower or deficiency in 59.4% ANLL cells and p53 gene deficience was frequent in M 4、M 5.Conclusion: Aberrant expression of gene was present in acute leukemia cells.The probe with 3 H dATP can be used in detecting gene expression.
出处
《河南医学研究》
CAS
1998年第4期345-346,共2页
Henan Medical Research
