摘要
为探讨重症肌无力的发病机理和乙酰胆碱受体抗体特异性与致病性的关系，用免疫印迹法测定了２株乙酰胆碱受体单克隆抗体的精细特异性．这２株抗体均能与硝酸纤维素膜上的乙酰胆碱受体γ和δ带结合，表明这２株抗体均不是抗致病性抗体识别的α亚单位主要免疫原区的抗体．这２株抗体对健康大鼠致病性的测定以及可变区的序列分析。
The authors made an investigation of the mechanism of myasthenia gravis and the fine specificity of antibodies against acetylcholine receptor(AChR) related to their pathogenicity. The two anti AChR monoclonal antibodies tested could bind to the γ and δ bands of AChR on nitrocellulose as shown by immunoenzymatic technique in Western blot,indicating that the two antibodies are not directed against the main immunogenic region of α subunit in AChR which is known the site recognized by pathogenic antibodies. The determination of the antibodies in inducing experimental autoimmune myasthenia gravis in rats and the sequence analysis of the antibody variable regions will reveal the relationship between the molecular structure and the pathogenicity of the antibodies.
出处
《延边大学医学学报》
CAS
1998年第4期200-203,共4页
Journal of Medical Science Yanbian University
