摘要
从8株酿酒酵母中初筛到一株2-苯乙醇产量达1.48g.L-1的菌株FD0419.以此为出发菌株,分别进行硫酸二乙酯化学诱变和原生质体紫外诱变,获得3株耐受性提高但2-苯乙醇产量下降的菌株,再与出发菌株进行原生质体融合.最终,筛得菌株R-UV3,其2-苯乙醇产量达2.51g.L-1,比出发菌株提高69.6%.
A strain which could produce 1.48 g·L-1 2-phenylethanol and was named FD0419,was screened out from eight Saccharomyces cerevisiae strains.Strain FD0419 and its protoplast were further treated with diethyl sulphate and ultraviolet respectively.Three mutants were attained which had higher tolerance but lower production,and then their protoplasts were fused with the protoplast of FD0419 respectively.Finally a fusant named R-UV3 was selected and could produce 2.51 g·L-1 2-phenylethanol that was 69.6% higher than the original strain FD0419.
出处
《福州大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第1期153-156,共4页
Journal of Fuzhou University(Natural Science Edition)
基金
福建省自然科学基金资助项目(2008J0150
2007F5069)
关键词
2-苯乙醇
酿酒酵母
原生质体
诱变
融合
2-phenylethanol
Saccharomyces cerevisiae
protoplast
mutagenesis
fusion
