摘要
为了探讨硒对富硒植物恩施碎米荠生理生化特性的影响,以Na2SeO3为硒源,设置0mg.L-1,15mg.L-1,30mg.L-1,45mg.L-1,60mg.L-1,75mg.L-1和90mg.L-1共7个不同质量浓度,探讨了水培条件下,硒对恩施碎米荠叶片抗氧化酶的影响.结果表明:硒质量浓度在15～90mg.L-1时,能促进叶片谷胱甘肽过氧化物酶(GSH-Px)的活性,酶活性与叶片含硒量在15～30mg.L-1呈正相关;≥30mg.L-1的硒处理能显著促进APX,CAT及POD的活性;SOD的活性在硒质量浓度为30～60mg.L-1时与对照组有显著性差异.促进恩施碎米荠叶片抗氧化酶活力的最适硒质量浓度是30～60mg.L-1.
The effects of selenium on the physiological-biochemical-characteristic in Cardamine enshiensis,which is a hyperaccumulator of selenium,was studied.The plant was cultivated in hydroponic culture without selenium and amended with increasing dosages of Na2SeO3 （15 mg·L-1,30 mg·L-1,45 mg·L-1,60 mg·L-1,75 mg·L-1 and 90 mg·L-1）.The leaves were analyzed by antioxidative enzymes.The results show that the activity of GSH-Px is promoted when selenium addition ranged from 15 mg·L-1 to 90 mg·L-1,significant increase in the enzyme activity is positively related to selenium content at 15～30 mg·L-1.The activities of POD,APX and CAT are significant increased at Se addition levels≥30 mg·L-1.Increase in the activity of SOD shows significant difference to the control group at selenium addition 30～60 mg·L-1.The optimum selenium addition is 30～60 mg·L-1,in which the antioxidative enzymes activity of leaves can be improved in Cardamine enshiensis.
出处
《华中科技大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2010年第3期116-119,共4页
Journal of Huazhong University of Science and Technology(Natural Science Edition)
基金
国家民委2007年度科研项目(07HB03)
湖北民族学院生物资源保护与利用湖北省重点实验室开放课题资助项目(2007001)
