摘要
目的探讨白细胞介素13基因Arg144Gln多态性与哮喘的相关性。方法选择178例小儿支气管哮喘患者为哮喘组,158例健康儿童为对照组,采用聚合酶链反应-限制性片段长度多态性方法检测白介素13(IL-13)基因第4外显子Arg144Gln多态性(rs20541)。结果哮喘组IL-13基因第4外显子rs20541位点CC、CT和TT基因型频率分别为40%、42%和18%,而对照分别为46%,44%和10%,差异具有统计学意义(P<0.05);TT基因型在哮喘组明显高于对照组,差异具有统计学意义(P<0.05)。结论IL-13基因第4外显子Arg144Gln多态性和哮喘的发生具有相关性。
Objective To explore the relationship between genetic Arg144Gln polymorphism and asthma in children.Methods 178 children with asthma and 158 healthy controls were recruited in this study.The Arg144Gln polymorphism （rs20541） was genotyped by PCR-RFLP methods.Results The genotype distributions of the control group and asthma group were in the Hardy-Weinberg equilibrium.The frequencies of CC,CT and TT were 40%、42% and 18% in asthma group while ones were 46%,44% and 10% in controls.There was significant difference in frequencies of TT genotype between controls and asthma cases.Conclusion Polymorphism in IL-13 gene might be a risk factor of asthma in children.
出处
《中国现代医药杂志》
2010年第3期46-47,共2页
Modern Medicine Journal of China
