摘要
目的 探讨甲状腺癌 p53基因甲基化状态.方法 用限制性内切酶HpaⅠ和MspⅠ酶切甲状腺癌及癌旁组织DNA,通过聚合酶链反应(PCR)扩增p53基因第5外显子,经琼脂糖凝胶电泳后,分析其电泳图谱.结果12例甲状腺癌中9例p53基因第5外显子出现高甲基化状态,而5例癌旁组织为低甲基化状态.结论p53基因高甲基化状态与甲状腺癌发生以及p53基因点突变有关.
PURPOSE To investigate the methylation of p53 gene in human thyroid carcinoma. METHODS The DNA of 12 thyroid carcinomas and 5 adjacent tissues of thyroid carcinoma were digested by restriction endonuclease enzymes Hap I and Msp I . Polymerase chain reaction (PCR) followed by agarose gel electrophoresis was used to detect the methylation in the exon 5 of p53 gene. RESULTS 5'-CCGG-3' site was methylated at the exon 5 of p53 gene in 9 thyroid carcinomas. However hypomethylation was found to exist in 5 adjacent tissues of thyroid carcinoma. CONCLUSION Hypermethylation of p53 gene plays an important role in thyroid carcinogenesis and the mutation of p53 gene.
出处
《中国癌症杂志》
CAS
CSCD
1998年第4期280-282,共3页
China Oncology
基金
国家自然科学基金课题
