摘要
目的研究桂枝汤在肠道不同部位的吸收特征。方法采用大鼠肠外翻模型,HPLC检测不同浓度桂枝汤中甘草苷、芍药苷、肉桂酸和甘草酸在肠道不同部位的吸收浓度,计算各成分的吸收参数,并分析它们在肠道不同部位的吸收特征。结果不同浓度桂枝汤中甘草苷、芍药苷、肉桂酸和甘草酸在各肠道均为线性吸收,R2均大于0.9,符合零级吸收速率;甘草苷在十二指肠、空肠、回肠,芍药苷、肉桂酸和甘草酸在十二指肠、空肠、回肠和结肠中的吸收速率常数(Ka)均随桂枝汤浓度的增加而增加(P<0.05),符合被动吸收;甘草苷在结肠中的吸收速率常数(Ka)随桂枝汤给药浓度的增加没有差异,符合主动转运;桂枝汤中甘草苷的最佳吸收部位为结肠,芍药苷和甘草酸的最佳吸收部位为十二指肠,肉桂酸在十二指肠和回肠中吸收均较好。结论肠道不同部位对桂枝汤中不同成分的吸收具有一定差异,除甘草苷在结肠部位为主动吸收外,所有被测成分在不同肠道均为被动吸收形式。
Objective To study the absorption of Guizhi decoction at different intestinal section. Methods In-vitro everted gut model was applied. The absorption concentration of liquiritin, paeoniflorin, cinnamic acid and glycyrrhizic acid, which are representative compositions of the Guizhi decoction, were detected by HPLC, and the absorption pa- rameter was calculated for describing the absorption character. Results The absorption curve of liquiritin, paeoni- florin, cinnamic acid and glycyrrhizic acid from different dosages of Guizhi decoction were linear. The K~ of liquiritin in the duodenum, jejunum and ileum, as well as paeoniflorin, cinnamic acid and glycyrrhizic acid in the duodenum, je- junum ileum and colon, increased along with the raised dosage of the Guizhi decoction （P 〈 0.05） , indicating a pas- sive absorption manner. The Ka of liquiritin in the colon was invariable along with the raised dosage, indicating an ac- tive transport. The absorption of liquiritin in the colon was better than other intestines, while paeoniflorin and gly- cyrrhizic acid can be absorbed better in duodenum, cinnamic acid can be absorbed better in both the duodenum and ileum. Conclusion Except that liquiritin is in active absorption in the colon, the absorption of liquiritin, paeoniflorin, cinnamic acid and glycyrrhizic acid from different dosages of Guizhi decoction is in passive absorption.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2010年第2期167-170,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金资助项目(30572334)
关键词
肠外翻
桂枝汤
甘草苷
芍药苷
肉桂酸
甘草酸
肠吸收
Everted gut
Guizhi decoction
Liquiritin
Paeoniflorin
Cinnamic acid
Glycyrrhizic acid
Intestinal absorption
