摘要
以甲苯胺兰（ＴＢ）修饰碳糊微电极为基体，将葡萄糖脱氢酶（ＧＤＨ）用丝素蛋白膜固定于修饰微电极表面制成了生物传感器。在 ｐＨ ７．０的 ＮａＯＨ－ＮａＨ２ＰＯ４缓冲溶液中，烟酰胺腺嘌呤二核苷酸（ＮＡＤ＋）的浓度为 １．０４×１０－３ｍｏｌ／Ｌ的条件下，其响应电流与葡萄糖浓度在 １．０ ×１０－４～３．２×１０－３ ｍｏｌ／Ｌ范围内有良好线性关系；响应时间为２０ｓ；检测限为 ４．０× １０－５ ｍｏｌ／Ｌ。该传感器面积小，稳定性、重现性均好。
An amperometric glucose dehydrogenase biosensor is reported. It is made up of toluidine blue (TB) modified carbon paste microelectrode and enzyme film by glucose dehyrogenase immobilized on silk fibroin. In pH 7. 0 NaOH-NaH2 PO4 medium, under the concentration of nicotinamide adenine dinucleotid (NAD+ ) being 1 .04× 10-3 mol/L, the biosensor has a linear response in the range of 1 .0× 10-4 - 3 .2 × 10-3 mol/L for concentration of glucose. The response time is 20 s. The lower detection limit of microsensor is 4× 10-5 mol/L. The biosensor has advantages of small volume, better stability and repeatability.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1998年第9期1093-1096,共4页
Chinese Journal of Analytical Chemistry
关键词
微电极
葡萄糖脱氢酶
化学修饰电极
传感器
Microelectrode, glucose, glucose dehydrogenase, carbon paste electrode, modified electrode
