摘要
采用压力超负荷心肌肥厚模型，以Ｆｕｒａ－２／ＡＭ作为荧光指示剂，观察Ａｍｉｌｏｒｉｄｅ（Ａｍｉ）和Ｅｎａｌａｐｒｉｌ（Ｅｎａ）预防性给药对压力超负荷左室肥厚（ＬＶＨ）大鼠心肌肥厚的形成及心肌细胞［Ｃａ２＋］ｉ的影响。结果表明，压力超负荷时，左心室重与体重之比（ＬＶＷＷ／ＢＷ）明显增加（Ｐ＜０．０１），左室心肌细胞内确有钙超载现象；Ａｍｉ和Ｅｎａ组的ＬＶＷＷ／ＢＷ较ＬＶＨ组明显降低（Ｐ＜０．０１），左室心肌细胞［Ｃａ２＋］ｉ亦明显降低（Ｐ＜０．０１）。给予ＫＣｌ４０ｍｍｏｌ·Ｌ－１、ＮＥ２０μｍｏｌ·Ｌ－１，预防给药组左室心肌细胞［Ｃａ２＋］ｉ的增加值明显低于ＬＶＨ组。
The preventive effect of Amiloride on the ratio of left ventricular wet weight and the body weight (LVWW/BW)of the LVH rats induced by partial ligation of abdominal aorta, [Ca 2+ ] i in the single myocardial cells was studied by a system of measurement of AR-CM-MIC, using Fura-2/AM as a fluoresent indicator. The LVWW/BW was increased markedly in the LVH group, and that of Ami,Ena treated group was lowered significantly than that of LVH group. The resting [Ca 2+ ] i of the LVH group was increased significantly compared with that of the control group, but the [Ca 2+ ] i of the Ami and Ena group was decreased markedly than that of the LVH group. In the presence of (KCl40 mmol·L -1 , NE 20 μmol·L -1 ), the elevation of [Ca 2+ ] i in the control and treated group was markedly lower than that of the LVH group. The result suggested that Ami may be a good drug for the treatment of the pressure-overloaded LVH,which was similar with that of Ena.
出处
《南通医学院学报》
1998年第4期419-421,418,共4页
ACTA Academiae Medicinae Nantong
基金
江苏省教委自然科学基金
