摘要
研究了纳米TiO2分离富集水样中痕量镉的最佳反应条件,应用自制抗Cd(Ⅱ)-iEDTA(Isothiocya-nobenzyi-EDTA)螯合物的单克隆抗体,建立了快速检测环境水样中重金属镉残留的胶体金免疫层析法。采用纳米钛富集水样中的痕量镉,用洗脱剂将吸附的镉离子洗脱后,再采用胶体金免疫层析法快速判断镉离子浓度,进而分析水样中的重金属镉含量。结果表明,pH 9.0时,Cd可被纳米TiO2定量富集,吸附于纳米TiO2上的镉离子可用0.1 mol/L的EDTA.2Na(乙二胺四乙酸二钠)溶液定量脱附。在优化实验条件下,纳米TiO2对Cd的吸附容量为14.7 mg/g,富集倍数可达50倍。制备了比色法判定结果的胶体金试纸条,并建立了纳米TiO2富集-胶体金试纸条联用检测方法。对实测样品的检测耗时约90 min,该方法对Cd的定量下限可达5μg/L,适用于环境水样的检测。
Based on our previous cadmium complete antigen coupling technology and anti-Cd（ Ⅱ ） - iEDTA（ Isothiocyanobenzyi -EDTA） monoclonal antibody, the immunocolloidal gold test strip was prepared and used for the detection of Cd （ Ⅱ ） in water samples. To improve the lower limit of quantification, Cd（ Ⅱ ） was preconcentrated with nanometer titanium dioxide and eluted with 0. 1 mol/L EDTA · 2Na quantitatively at pH 9.0. Under optimal conditions, the adsorption capacity of nano- TiO2 for Cd（ Ⅱ ） was found to be 14.7 mg/g and the maximum preconcentration factor was 50. The lower limit of quantification for Cd was found to be 5 μg/L. The results obtained by the proposed method were in conformity with those obtained by ICP- AES method.
出处
《分析测试学报》
CAS
CSCD
北大核心
2010年第3期247-251,共5页
Journal of Instrumental Analysis
基金
"863"国家高技术研究发展计划资助项目(2008AA10Z427)
广州市天河区科技计划资助项目(085G079)
关键词
纳米TIO2
镉
胶体金
吸附
nano-TiO2
cadmium
colloidal gold
adsorption
