摘要
目的:寻找在监测再生障碍性贫血(再障)患者环孢素A(CsA)浓度时高效液相色谱(HPLC)法和多克隆荧光偏振免疫(PFPLA)法两者之间的差异和相关住.方法:建立一种特异性测定再障患者服药后全血中CsA_(原药)的HPLC法.并与非特异性测定CsA原药及其代谢物总浓度的PFPIA法比较.结果:两者测定值.经配对t检验P<0.01,经相关与回归分析里线性关系,方程为:Y_(PFPLA)=2.47X_(HPLC)+69.52,r=0.9124,n=38,(P<0.01).结论:监测再障患者CsA血药浓度较理想的方是既测定CaA_(原药+代谢物)总浓度,又要同时测定CsA_(原药)浓度,并计算出CsA_(原药+代谢物)/CsA_(原药).从这一比值的变化表明患者可能肝功能异常或者正在使用药酶诱导刊或药酶抑制剂,借以调整治疗方案.
AIM : To find the difference and comparison between HPLC and polyclonal flu-o-rescence polarization immunoassay (PFPIA) which determined cyclosporine A(CsA) in aplastic anemia (AA) patients. METHODS: A specific HPLC method for determination of whole blood concentration of parent CsA in AA patients receiving CsA was established and compared with nonspecificity PFPIA for determination of the summation of parent CsA and metabolites. RESULTS:Both determination value was tested by t-test for dependent sample (P<0. 01). Both determination value was analysed by correlation and regression. HPLC and PFPIA methods had a line relation. The curve equation was Y_(PFPIA) = 2. 47X_(HPLC) + 69. 52, r = 0.912 4,n = 38,(P<0. 01). CONCLUSION:It is clear that the good method for determintion of whole blood concentration of CsA is both the summation of CsA_(parent+metabolites) and CsA_(parent), Then the ratio was calculated. The variety of the ratio CsA_(parent+metabolites)/CsA_(parent) shows that the patient's liver was abnormal or the patient had administered enzyme inductor or enzyme inhibitor,in order to adjust projects.
出处
《中国临床药学杂志》
CAS
1998年第6期282-285,共4页
Chinese Journal of Clinical Pharmacy
关键词
环孢素A
再生障碍性贫血
血药浓度
HPLC
PFPIA
high performance liquid chromatography
polyclonal fluorescence polarization immunoassay
cyclosporine A
metabolites
aplastic anemia
blood concentration
