摘要
首次研究了meso-四(4-磺基苯)卟啉和锰的络合物(MnTPPS_4)作为过氧化物酶模拟酶对高香草酸(HVA)和H_2O_2荧光反应的催化作用,拟订了用模拟酶测定痕量H_2O_2和葡萄糖的荧光测定方法,并对模拟酶和天然酶的性能进行了比较。用MnTPPS_4测定H_2O_2和葡萄糖的灵敏度分别可以达到8.5×10^(-8)mol/L的H_2O_2和0.5μgmL^(-1)的葡萄糖。
A fluorescence reaction of homovanillic acid with hydrogen peroxide catalyzed by mimetic enzyme of peroxidase (manganese tetrakis(sulfophenyl) porphyrin) was investigated. A high sensitive fluorometric method for the determination of hydrogen peroxide and glucose has been developed. The sensitivities of the determination of hydrogen peroxide and glucose are 8.5 × 10-8mol/L for hydrogen peroxide and 0.5μg·mL-1 for glucose, respectively. The recommended method was applied to determine hydrogen peroxide in snow and glucose in human serum and satisfactory results were obtained.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1989年第3期229-232,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
MnTPPS4
HVA
H2O2
荧光反应
Mimetic enzyme of peroxidase, Determination of hydrogen peroxide by fluorescence method, Glucose
