摘要
目的：观察川芎嗪对心肌细胞缺氧缺糖损伤的保护作用。方法：本实验利用心肌细胞缺氧缺糖损伤模型，采用同位素掺入、Ｎｏｒｔｈｅｒｎ杂交分析方法，观察川芎嗪注射液对心肌细胞缺氧缺糖时蛋白质、ＲＮＡ合成及一氧化氮合酶（ＮＯＳ）ｍＲＮＡ表达的影响。结果：川芎嗪注射液可提高培养心肌细胞缺氧缺糖时３Ｈ亮氨酸（Ｌｅｕ）和３Ｈ尿嘧啶核苷（ＵＲ）的掺入率，促进蛋白质、ＲＮＡ合成，诱导ＮＯＳｍＲＮＡ在缺氧缺糖心肌细胞的表达。
OBJECTIVE:To investigate the protective effects of ligustrazine on cultural myocardial cells against hypoxia and lack of sugar.METHOD:This experiment adopted approaches of isotopic incorporation and Northern hybridization to examine the effects of ligustrazine on synthesis of protein and RNA,and NOS mRNA expression in cultural myocardial cells against hypoxia and lack of sugar.RESULTS:ligustrazn can improve the incorporation ratio of 3H Leu and 3H UR,enhance the synthesis of protein and RNA,and induce the expression of NOS mRNA in myocardial cells against hypoxia and lack of sugar.CONCLUSION:ligustrazine can improve the protective capability of myocardial cells to the injury of hypoxia and lack of sugar.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1998年第12期724-726,共3页
Chinese Pharmaceutical Journal
关键词
川芎嗪
缺氧
缺糖
心肌损伤
蛋白质
RNA
ligustrazine
myocardial cell
injury of hypoxia and lack of sugar
