摘要
利用亚洲首个开放式臭氧浓度升高(Ozone-free air controlled enrichment)平台,以武运粳21(粳稻)和两优培九(杂交稻)两个耐性不同的水稻品种为材料,研究了大气臭氧浓度升高对水稻叶片乙醇酸氧化酶(GO)、多酚氧化酶(PPO)和抗坏血酸氧化酶(AAO)3种呼吸作用相关酶的影响,初步探讨了高臭氧浓度条件下水稻不同基因型对臭氧胁迫响应机制的差异性。结果表明:臭氧处理67天,促进了GO、PPO和AAO的活性,武运粳21升高的幅度(24.6%、9.25%和67.8%)大于两优培九的升高幅度(11.18%、7.26%和36.85%);在处理79天,臭氧胁迫抑制了水稻叶片GO、PPO和AAO的活性,品种间比较,武运粳21的3种酶降低的幅度(21.18%、6.14%和31.87%)小于两优培九(42.7%、33.93%和51.1%)。结果说明,武运粳21在通过提高3种酶的活力而进行较强的呼吸作用,起到耗散过剩光能并保护光合器官的作用,从而缓解臭氧对光合器官的损害缓解臭氧对水稻叶片造成的伤害,因此武运粳21比两优培九对臭氧耐受性强。
In this paper, two rice varieties（Wuyunjing21 and Liangyoupeijiu） were used to detect polyphenol oxidase （PPO）, ascorbic acid oxidase （AAO）, glycolic acid oxidase （GO） activities of leaves for heading date of rice （Oryza sativa L.） under the ambient atmosphere 03 concentration （Ambient） and O3 free air concentration enrichment （O3-FACE） conditions. Results showed that： elevated 03 concentration enhance the activities of PPO, AAO and GO after 67 days 03 treatment, Wuyunjing 21 increased these enzymes activity much than those of Liangyoupeijiu; however, after 79 day 03 treatment, elevated O3 concentration decreased GO, PPO and AAO activity, especially these enzymes activity reduction of Liangyoupeijiu were much than those of Wuyunjing 21. The results indicated that, Wuyunjing 21 have strong respiration by enhancing three respiration enzymes activity to dissipate of excess light energy and protect the photosynthetic apparatus for alleviating the damage of ozone on the photosynthetic apparatus of rice leaves. So we concluded that Liangyoupeijiu was more susceptible to ozone than Wuyunjing 21.
出处
《中国农学通报》
CSCD
北大核心
2010年第6期260-264,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"臭氧升高条件下作物臭氧通量-光合响应关系研究"(30770408)
国家科技部国际科技合作计划项目(2009DFA31110)
中国科学院科研装备研制项目(YZ0603)
中国科学院国际合作重点项目"近地表臭氧浓度升高对农田生态系统的影响"(GJHZ0748)
中国科学院南京土壤研究所知识创新工程领域前沿项目"臭氧浓度升高条件下的作物损伤与适应机制研究"(ISSASIP0709)
日本环境厅全球环境研究基金项目(中日合作)"近地表臭氧浓度升高对东亚的影响预测-植被和农作物损伤风险"(C-062)~~
