摘要
创建粳稻新的种质资源,对加速粳稻遗传育种研究具有重要意义。以粳稻品种秀水11、日本晴为材料,利用30Gy60Coγ-射线辐照其成熟胚愈伤组织筛选突变体。在MR2代群体中观察到叶片、茎秆、籽粒和穗等形态性状发生变异的突变体以及育性和生育期等生理性状方面的变异株;这些突变体为水稻新品种选育和功能基因组学研究提供了良好的基础材料。γ-射线辐照粳稻愈伤组织,利用核诱变与体细胞无性系变异相结合方法,增加再生植株中有利突变类型,其中一些突变体在水稻生产上有较高的应用价值。
Creation of new germplasm derived from japonica rice can accelerate genetic breeding of japonica rice. Callus induced from mature embryos of japonica rice varieties ＂xiushuill＂ and ＂Nipponbare＂ were irradiated with 60Coγ-rays at the dosage of 30 Gy. Morphological variations including leaf, stalk, seed, panicle at vegetative and reproductive stages and various physiological characters were observed in MR2 generation. These mutants were helpful for gene identification and gene functional analysis as well as breeding of new rice variety. The integral application of somaclonal variation technique and irradiation treatment induced more useful mutants in the present study. Some variant plants showed high potential in rice production.
出处
《中国农学通报》
CSCD
北大核心
2010年第6期39-42,共4页
Chinese Agricultural Science Bulletin
基金
安徽省农业科学院院长青年创新基金(06A1101)
农业部农业公益性行业科研专项"核技术农业应用"(200803034)
