摘要
目的研究BMI-l基因在儿童急性白血病患者中的表达及临床意义。方法收集2008年7月1日-2009年4月30日郑州大学第三附属医院和郑州市其他医院的初诊急性白血病患儿标本46例,对照组标本30例。实验经患儿监护人知情同意,并经医院伦理委员会批准。运用反转录-聚合酶链反应(RT-PCR)检测其外周血BMI-1 mRNA表达水平,应用SPSS12.0软件进行数据分析。结果1.BMI-l基因在儿童急性白血病中的表达水平显著高于对照组(P<0.05);2.粒细胞性白血病中该基因部分表达或弱表达,而各型B淋巴细胞性白血病中均有表达,且高于粒细胞性白血病(P<0.05);3.联合化疗未缓解组其表达水平高于缓解组,但二者比较差异无统计学意义(P>0.05),缓解后均未检测到BMI-1 mRNA;4.初治组、复发组与完全缓解组比较,前二者BMI-1 mRNA表达均显著高于后者(P<0.05);初治组、复发组与对照组比较差异有统计学意义(P<0.05),完全缓解组与对照组比较差异无统计学意义(P>0.05)。结论BMI-1基因在儿童急性白血病中表达增高,完全缓解者该基因表达水平趋于正常,提示BMI-1基因可能参与急性白血病的发生、发展过程,并可作为急性白血病的发病、复发及治疗和预后判断的指标之一。
Objective To study the expression of BMI-l gene in children with acute leukemia and its clinical significance.Methods The clinical specimens of 46 children with acute leukemia who were diagnosed lately in the Third Affiliated Hospital of Zhengzhou University and other hospitals in Zhengzhou from Jul.1,2008 to Apr.30,2009 were collected,while peripheral blood specimens of 30 healthy children were collected as control group.With the guardians′ informed consent,the experiment was approved through the hospital ethics committee.The level of BMI-1 mRNA′s expression was tested using reverse transcription-polymerase chain reaction（RT-PCR）,while data were analyzed through the application of SPSS 12.0 statistical software.Results 1.The level of BMI-l gene′s expression in children with acute leukemia was significantly higher than that in control group（P〈0.05）;2.Partial expression or weak expression of the gene in children with myelogenous leukemia was found,while universal expression was found in various types of B-lymphocytic leukemia,which was higher than that in myelocytic leukemia group（P〈0.05）;3.The level of BMI-1 gene′s expression was higher in chemotherapy non-remission group than that in remission group,but the difference was not statistically significant（P〉0.05）,after complete remission,BMI-1 mRNA was not detected in the 2 groups;4.Compared with the complete remission group,expression of BMI-1 mRNA in the untreated group and the recurrence group was significantly higher（P〈0.05）;and expression of BMI-1 mRNA in the former two was significantly different with that in control group（P〈0.05）;while there was no significant difference between the complete remission group and the control group（P〉0.05）.Conclusions BMI-1 gene was highly expressed in children with acute leukemia,and the level of the gene expression in patients of complete remission normalized,which suggests that the gene may be involved in the occurrence and the development process of leukemia;therefore,it is possible to regard the gene as a molecular marker to evaluate the development,relapse and prognosis of the patients with acute leukemia.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2010年第3期198-200,共3页
Journal of Applied Clinical Pediatrics
关键词
BMI-1基因
急性白血病
反转录-聚合酶链反应
儿童
B - cell - specific moloney murine leukemia virus insertion site 1 gene
acute leukemia
reverse transcription polymerase chain reaction
child
