摘要
研究了洗脱剂流速、负载量和硅胶初始含水量对硅胶柱层析分离纯化胭脂虫红色素的影响，得到了柱层析的优化工艺条件：硅胶初始含水量在10％-14％之间，洗脱剂流速为5ml/min，负载量为0．9g／100g硅胶。在优化条件下胭脂虫红色素经过硅胶层析后回收率在75％以上，纯度有明显改善，可达54．37％。
This paper studied the effects of flow rate, sample load and initial water content in silica gel on the purification of cochineal dye. The results showed that the optimal conditions for the extraction were obtained as follows ： the initial water content in silica gel was in the range of 10%-14% ,flow rate 5 mL/min,sample load 0.9 g/100 g. After the process of silica gel column chromatography,the recovery of cochineal dye may be more than 75% and the content of carminic acid can reach 54.37%.
出处
《天然产物研究与开发》
CAS
CSCD
2009年第B10期383-385,共3页
Natural Product Research and Development
基金
国家科技支撑计划（2006BAD06807）
关键词
胭脂虫红色素
柱层析
硅胶
精制
cochineal dye
column chromatography
silica gel
purification
