摘要
目的:探讨补肾活血汤防治绝经后骨质疏松症的机理,为补肾活血汤的临床应用提供实验依据。方法:将60只雌性SD大鼠用去势的方法建立绝经后骨质疏松症模型,另将12只雌性SD大鼠同法手术暴露卵巢而不切除。造模后将60只去势大鼠随机分为模型组(OvX)、高剂量组(H-iso)、中剂量组(M-iso)、低剂量组(L-iso)、阳性对照组(E2)5组,另外12只列入假手术组(Sham)。Sham组和OvX组以蒸馏水灌胃;H-iso组、M-iso组和L-iso组分别以不同剂量补肾活血汤灌胃;E2组用尼尔雌醇灌胃。治疗12周后检测各组大鼠尿肌酐、尿脱氧吡啶酚、骨生物力学指标、骨密度以及整合素αVβ3的表达。结果:补肾活血汤各剂量组及E2组与模型组相比,骨密度、骨强度、尿脱氧吡啶酚、尿脱氧吡啶酚/尿肌酐水平及整合素αVβ3的表达明显改善,差异有统计学意义(P<0.05)。结论:补肾活血汤能有效提高骨密度和骨强度,通过降低尿脱氧吡啶酚、尿脱氧吡啶酚/尿肌酐水平以及破骨细胞整合素αVβ3的表达抑制骨吸收,从而达到治疗绝经后骨质疏松症的目的。
To explore the mechanism of Kidney-Supplementing and Blood-Quickening Decoction（KSBQD） on the preventive treatment of postmenopausal osteoporosis, on purpose of providing experimental evidence for the clinical application of KSBQD. Methods ：60 female SD rats were administrated with ovariectomy for establishing models of postmenopausal osteoporosis, while ovarians of other 12 female SD rats were exposed but were not removed. After molding,60 ovariectomized rats were randomly divided into 5 groups as model group（ OvX group）, high dose group （H-iso group）, middle dose group （M-iso group ）, low dose group （L-iso group ）and positive control group（ E2 group） ,while the other 12 rats were divided into sham-operated group（ Sham group）. Rats in Sham group and OvX group were lavaged with distilled water;Rats in H-iso group,M-iso group and L-iso group were lavaged with various doses of KSBQD respectively,Rats in E2 group were lavaged with nylestriol. After 12 weeks treatment, urine creatinine （Ucr） , urine deoxypyridinoline （UD） , indicators of bone biomechanics（IBB） ,bone mineral density（BMD） and the expression of integrin αVβ3 were detected in each group. Results： BMD, bone strength,the level of UD,the level of UD/Ucr and expression of integrin αVβ3 of H-iso, M-iso, L-iso and E2 groups were improved much more obviously than that of OvX group. There was statistical difference between these groups （ P 〈 0.05 ）. Conclusion： KSBQD improves BMD and bone strength effectively, and it can hold-up bone resorption by lowering the level of UD, UD/Uer and expression of osteoelast integrin αVβ3, so as to achieve the purpose of treating postmenopausal osteoporosis.
出处
《中医正骨》
2010年第2期3-6,共4页
The Journal of Traditional Chinese Orthopedics and Traumatology
关键词
骨质疏松
绝经后
补肾活血汤
破骨细胞
Osteoporosis, postmenopausal
Kidney-Supplementing and Blood-Quickening Decoction
osteoclast
