摘要
以莲瓣兰杂交种子原生地共生萌发突破种皮的胚为试验材料,采用正交试验设计研究次氯酸钠浓度、灭菌时间、灭菌次数及超声波处理时间对莲瓣兰胚培养过程中真菌污染控制及胚培养情况的影响。结果表明:莲瓣兰胚培养过程中以次氯酸钠浓度∶灭菌时间∶灭菌次数∶超声波处理时间(4%∶15 min∶1次∶6 min)的配比胚被真菌污染的污染率最低为16.0%,但是胚成活率较低仅为14.3%,不宜作为莲瓣兰胚培养的灭菌条件;经过正交试验设计优选出莲瓣兰胚培养过程中胚成活率作用大小为次氯酸钠浓度>超声波处理时间>灭菌时间>灭菌次数,以次氯酸钠浓度∶灭菌时间∶灭菌次数∶超声波处理时间(1%∶20 min∶2次∶3 min)的配比胚成活率高达93.3%,同时胚被真菌污染率仅为19.00%,可以作为莲瓣兰(Cymbidium torliseplum)杂交种子原生地共生萌发突破种皮的胚培养灭菌条件,在此条件下胚能够从培养基中吸收营养物质,经过培养胚生长发育成根状茎。
The experiment material is embryo of Cymbidium torliseplumin in hybrid seeds in suit when symbiotic germination breaks through the coat of seed. Orthogonal experimental design was used to observe the effect of sodium hypochlorite concentration, sterilization time, sterilization times and ultrasonic treatment time on the control of fungi pollution and embryo culture. The results showed that the ratio of embryos contaminated by fungi was 16.0% ,with the lowest embryo survival rate of 14. 3%. The ratio of sodium hypochlorite concentration ： sterilization time ： sterilization times ： ultrasonic treatment time （ 4% ： 15 min ： 1 times ： 6 rain）. Obviously, it could not suit the sterilization condition of the embryo culture. Furthermore, we noticed that the contribution on the embryo survival rate ordered by decreasing was sodium hypoehlorite concentration, sterilization times, ultrasonic treatment time, and sterilization time. The embryo survival rate was 93.3%0 ,and the contamination rate was just 19.0%. The concentration of sodium hypochlorite ＂ sterilization time .＂ sterilization times ： ultrasonic treatment time（= 1% ： 20 min ： 2 times ： 3 min）. It demonstrated that this matching can be used to cultivate the Cymbidium torliseplum embryo ,and under this condition,embryo can absorb nutrients from the culture medium, and develop into rhizome.
出处
《北方园艺》
CAS
北大核心
2010年第4期129-131,共3页
Northern Horticulture
基金
云南省大理州科技局及大理学院药物研究所资助项目(2009yy02)
关键词
莲瓣兰
胚培养
正交设计
真菌污染
Cymbidium torliseplum
embryo culture
orthogonal test
fungi pollution
