摘要
目的评价间质巨噬细胞（ＩＭ）对成纤维细胞增殖的影响。方法在成纤维细胞培养液中分别加入肺纤维化大鼠肺泡巨噬细胞（ＡＭ）、ＩＭ培养上清培养。结果实验组第１天两种巨噬细胞的促增殖作用即开始升高，第７天达高峰（ＡＭ３３２％，ＩＭ３４２％，Ｐ＜０．０１）；而ＩＭ和ＡＭ培养上清中肿瘤坏死因子α（ＴＮＦα）、血小板源生长因子（ＰＤＧＦ）与成纤维细胞增殖活性水平成显著相关（ｒ＝０．６５７，０．７１３，Ｐ＜０．０１）。结论不仅ＡＭ，而且ＩＭ也能释放促成纤维细胞体外增殖的细胞因子。由于ＩＭ与成纤维细胞紧密相邻，对纤维化的形成可能更具有意义。
Objective To evaluate the role of interstitial macrophages (IMs) in modulating the proliferative capacity of fibroblast(FB). Method IMs isolated by collagenase IA digestion of disaggreted lung tissues from bleomycin treated rats were studied in this regard.The culture supernatants of alveolar macrophages(AMs) and of IMs were added to culture media of FBs. Result The releases of growth factors by the two sub populations of macrophage were already elevated on 1st day following bleomycin instillation, and peaked on 7th day at a level nearly 3 fold above that of control groups(IM 342%, AM 332%, P <0.01). the proliferative capacity was correlated well with the levels of TNFα and PDGF ( r =0.657,0.713, P <0.01). Conclusion Not only AMs, but also IMs could release growth factors to modulate the proliferation of FBs in vitro. IMs might be of more signicance in maintenance of the process of pulmonary fibrosis because of its proximity to FBs.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1998年第11期686-688,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
