摘要
目的 :动态观察博莱霉素致肺纤维化大鼠间质巨噬细胞 ( IM)释放 IL- 1和 TNFα的变化。方法 :酶消化法分离间质巨噬细胞 ,生物活性法测定 IL- 1和 TNFα。结果 :博莱霉素注入后肺泡巨噬细胞 ( AM)分泌的IL- 1未发生变化 ;而 IM则在第 1天出现短暂的分泌高峰 ( 2 80 .2 4 U/ 1 0 6IM)。 AM和 IM释放的 TNFα都在第 3天明显升高 ( AM33.33% ,IM2 2 .0 1 % ) ,第 7天达高峰 ,AM的分泌峰值明显高于 IM( AM69.1 3% ,IM46.72 % )。结论 :AM和 IM释放细胞因子的功能存在一定差异 ,它们在肺纤维化中可能发挥不同的作用。
Objective:To study the interstitial macrophages(IMs) release of IL-1 and TNFα in pulmonary fibrosis rats.Methods:The IMs were isolated from post lavaged rat lungs by enzymatic digestion of tissue.The levels of cytokines were assessed by bioassay.Results:There were no changes in IL 1 release by alveolar macrophages(AMs),whereas an early transient peak resease by IM after bleomycin treatment (280.24U/10 6IM).Both AMs and IMs could release TNFα,which elevated on 3rd day (AM 33.33%,IM22.01%) and peaked on 7th day.The peak value of TNFα released by AMs was significantly higher than that released by IMs.Conclusion:IM and AM are functionally different in releasing cytokines in the process of fibrosis.
出处
《滨州医学院学报》
2000年第2期105-107,共3页
Journal of Binzhou Medical University
