摘要
目的探讨职业苯接触与尿中反一反式黏糠酸（ttMA）和苯巯基尿酸（SPMA）的相关性，评定两接触标志物作为生物监测指标的适用性。方法对44名制鞋厂接苯工人进行个体苯暴露水平的作业环境监测，采集当日班前与班后尿样，分别用高效液相色谱和液质联谱测定尿中ttMA和SPMA含量。结果个体苯接触浓度为2．57～146．11mg／m^3，几何平均浓度为（27．91±3．29）mg／m^3。班后尿中ttMA和SPMA含量均较班前增高，差异有统计学意义（P〈0．01），班后ttMA和SPMA与空气苯浓度的相关系数分别为0．905（P〈0．01）和0．537（P〈0．01），个体苯接触代谢转化为ttMA和SPMA的相对内暴露指数（RIE）随苯接触浓度的增高而下降。结论在中、高浓度的苯接触时，班后尿ttMA与空气苯浓度的相关性优于SPMA。
Objective To investigate correlation of occupational benzene exposure with urinary trans, trans-muconic acid （ttMA） and S-phenylmercapturic acid （SPMA）, and assess the applicability of biomarkers in bio-monitoring of benzene exposure. Methods Benzene exposure was measured in 44 shoe-makers and air samples were collected with personal samplers. Pre-shift and post-shift urine samples were collected for ttMA determination by HPLC and for SPMA measurement by HPLC-MS. Results The personal benzene exposure ranged from 2.57 mg/m^3 to 146.11 mg/m^3 with geometric mean value of （27.91±3.29） mg/m^3. Both levels of urinary ttMA and SPMA in post-shift were higher than those in pre-shift （P〈0.01） and were significantly correlated with personal benzene exposure （Pearson r= 0. 905 ,P〈0.01 and r=0. 537 ,P〈0.01, respectively）. The relative internal exposure index （RIE） of benzene bio-transformed metabolites decreased with increasing of the benzene concentration. Conclusions The correlation of post-shift urinary ttMA concentrations with benzene exposure levels is superior to that of SPMA in moderate and high levels of benzene exposure.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2010年第1期19-22,共4页
Industrial Health and Occupational Diseases
关键词
苯
生物监测
反—反式黏糠酸
苯巯基尿酸
Benzene
Biological monitoring
Trans, Trans-muconic acids S-phenylmercapturic acid
