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猪CACNA1S基因部分序列多态性检测及其与肉质性状的关联分析 被引量：3

Polymorphism of Partial Sequence of the Porcine CACNA1S Gene and Its Association with Meat Quality Traits
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摘要 采用PCR-SSCP技术,对30头苏钟猪和97头金华与皮特兰杂交F2代(JPF2)猪CACNA1S基因进行多态性检测,结合肉质性状作关联分析。结果表明:在猪CACNA1S基因1 234 bp核酸序列中,检测到25个单核苷酸突变点(SNP);关联分析结果显示,引物1S-65的AA、BB基因型对后腿肌肉重存在显著影响(P<0.05),AB基因型对后腿pH存在显著影响(P<0.05);引物1S-66的BB、BC基因型对蹄重存在极显著影响(P<0.01)。 Single-nucleotide polymorphism of CACNA1S gene of 30 Suzhong pigs and 97 F2 offspring of Jinhua and Pietran pigs was analyzed by polymerase chain reaction-single strand conformational polymorphism（PCR-SSCP） ,and its association analysis with meat quality traits was conducted. In results, 25 mutations were found in 1 234 bp of CACNAIS gene. It was observed by association analysis that the AA and BB genotypes of primer 1S-65 had significant effects on ham weight （ P〈0.05 ）, the AB genotype of primer 1 S-65 had significant effects on ham pH （ P〈0. 05 ）, and the BB and BC genotype of primer 1 S-66 showed the most significant effects on hoofs weight （P〈0. 01 ）.
作者 王雪 方晓敏 武玮 刘源 任守文 刘红林
机构地区 江苏省农业科学院畜牧研究所 南京农业大学动物科学院
出处 《江苏农业学报》 CSCD 北大核心 2010年第1期101-106,共6页 Jiangsu Journal of Agricultural Sciences
基金 国家资源平台项目(2005DKA2101) 江苏省高新技术研究项目(BG2005305) 江苏省农业科学院博士后基金项目(6510607)
关键词 猪 CACNA1S基因 PCR—SSCP 肉质性状 相关分析 porcine CACNA1S gene PCR-SSCP meat quality trait association analysis
分类号 S828.8 [农业科学—畜牧学]
  • 相关文献

参考文献17

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二级参考文献40

  • 1方晓敏,赵晓枫,郭晓令,徐宁迎.应用辐射杂种细胞系对猪CACNA1S基因的定位研究[J].畜牧兽医学报,2006,37(3):309-312. 被引量：7
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江苏农业学报
2010年 第1期

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