摘要
采用核磁共振氢谱法(1H NMR)测定了苏丹红Ⅰ及苏丹红Ⅱ标准物质的纯度。分别以国家一级标准物质苯甲酸(GBW 13233)和国家二级标准物质尼泊金乙酯(GBW(E)100064)为内标,以氘代氯仿为溶剂,使用B ruker AVANCE DRX-500 NMR仪进行检测。实验优化了弛豫时间、扫描次数等定量参数,对定量峰的选择与积分进行了讨论。优化后的弛豫时间为30 s,扫描次数为32次;苏丹红Ⅰ、Ⅱ的纯度分别为96.1%、87.5%,其测量相对标准偏差分别为0.4%、0.6%。
A novel approach was developed for the determination of purities of Sudan red Ⅰ and Sudan red Ⅱ by proton-nuclear magnetic resonance（1H NMR） using deuterated chloroform as a solvent with two internal standards. Benzoic acid（a primary certified reference material of China（GBW13233） and ethylparaben（a secondary certified reference material of China（GBW（E） 100064）） were used as internal standards for each Sudan red, respectively. Experimental parameters of NMR were extensively investigated. Under the optimal conditions, the purity of Sudan red Ⅰ and Ⅱ were 96. 1% and 87.5% , respectively, and its relative standard deviations （RSDs） were 0. 4% and 0. 6% , respectively.
出处
《分析测试学报》
CAS
CSCD
北大核心
2010年第2期194-198,共5页
Journal of Instrumental Analysis
基金
中国计量科学研究院基本业务费资助项目(AKY0766)
关键词
核磁共振法
苏丹红
标准物质
nuclear magnetic resonance（NMR）
Sudan red
certified reference material
