摘要
目的:研究乳腺癌患者的癌组织和癌旁正常组织线粒体DNA(mtDNA)D-loop区碱基突变差异,以探讨mtDNA D-loop区碱基突变与乳腺癌发生发展的相关性。方法:采用聚合酶链反应和DNA测序相结合的方法,对20例乳腺浸润性导管癌患者癌组织和癌旁组织线粒体D-loop区进行扩增并测序分析。结果:11例乳腺癌患者癌组织mtDNA D-loop区存在突变,类型以点突变为主,同时发现在线粒体DNA的某些位点发生突变的频率明显较高。结论:mtDNA D-loop区碱基在癌组织中的突变率较癌旁正常组织显著增高,提示mtD-NA D-loop区碱基突变可能在乳腺癌发生发展中发挥重要作用。
OBJECTIVE：To investigate whether the mtDNA D-loop mutation plays a role in the invasive ductal breast cancer. METHODS：The matched distant normal and tumor tissues from 20 invasive ductal breast cancer patients were analyzed by PCR and DNA sequencing,with special attempt to characterize the spectrum of mtDNA D-loop somatic mutations and their potential significance in cancer diagnosis and development were evaluated. RESULTS：There were mtDNA D-loop mutations in 11 invasive ductal breast cancer tissues,and there were higher rate of mtDNA D-loop mutations in some place. CONCLUSIONS：There are higher rates of mtDNA D-loop somatic mutations in cancerous tissues compared with in distant normal tissues. Based on the mtDNA mutation pattern in tissues of breast cancer observed in this study,its concluded that mtDNA D-loop mutations play a role in the invasive ductal breast cancer.
出处
《中华肿瘤防治杂志》
CAS
2009年第24期1922-1924,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
首都医学发展科研基金(2007-2050)
十一五重大专项(2008ZX10001-007)
关键词
乳腺肿瘤
DNA
线粒体
突变
breast neoplasms
DNA
mitochondrial
mutation
