摘要
目的:探讨补肾益气活血方——葆青康对老年大鼠自由基、皮肤、免疫和性功能的影响。方法:采用电镜扫描观察不同年龄大鼠皮毛;采用TBA比色法观察不同年龄大鼠不同组织器官MDA变化情况;采用单向免疫扩散法和醋酸萘酯酶染色法测定老年大鼠血中IgG和外周血T-淋巴细胞百分率;用放免法测定血浆中睾酮和雌二醇含量。结果:葆青康能改善中老年大鼠皮毛衰老状态;大鼠心、脑、肝线粒体和微粒体MDA随龄增高,葆青康能降低老年大鼠心、脑、肝和全血中MDA含量;葆青康能提高中、老年大鼠胸腺和脾的脏器指数,提高中、老年大鼠血中IgG和外周血T-淋巴细胞百分率,增强中、老年大鼠的免疫力;葆青康能增加老年大鼠血浆中睾酮和雌二醇含量。结论:葆青康通过抗自由基、提高机体免疫力和增强机体性功能的方面的作用而具有抗衰老作用。
Objective：To explore the effects of Baoqingkang（葆青康） on free radical,skin,immunity and sexual function of the aged rat.Methods：The method of electron microscope scanning was employed to measure the effect on the fur of the various ages of rats;TBA method was employed to measure the change of MDA contents with the increased age in the various tissues and organs of rats.Single immunodiffusion technique and the staining method of α-naphthyl acetate esterase was employed to measure IgG and T-lymphocyte percentage of whole blood of the aged rats,respectively;Radio-immunity method was employed to measure the contents of testosterone and estradiol.Results：Baoqingkang can improve the status of aging skin fur of middle-aged rats and aged rats;Baoqingkang can decrease MDA contents in the brain,liver,heart and the whole blood.Baoqingkang can improve the thymus and spleen organ index,and increase IgG and T-lymphocyte percentage of whole blood,increase the immunity in middle-aged rats and aged rats;Baoqingkang can increase plasma testosterone and estradiol content,improve sexual function in old rats.Conclusion：Baoqingkang has anti-aging effect by improving anti-free radicals,enhancing the body immunity and enhancing sexual function.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2009年第6期77-80,共4页
Pharmacology and Clinics of Chinese Materia Medica
