摘要
目的研究普罗布考对2型糖尿病患者外周血单核细胞Toll样受体4/核因子κB信号转导通路的影响,以进一步探讨普罗布考抗动脉粥样硬化机制。方法选择85例2型糖尿病合并冠心病患者,随机分为对照组和治疗组:对照组按常规治疗,治疗组在常规治疗基础上加用普罗布考0.375 g,每天2次,口服,总疗程为24周。运用Real-time PCR、Western blotting方法分别测定治疗前后外周血单核细胞Toll样受体4、核因子κB mRNA和蛋白表达水平;ELISA检测外周血血清中白细胞介素1β的表达水平。结果普罗布考显著降低2型糖尿病合并冠心病患者总胆固醇、甘油三酯、低密度脂蛋白胆固醇和氧化型低密度脂蛋白水平,显著下调外周血单核细胞Toll样受体4 mRNA和蛋白的表达(P<0.05),明显减少核因子κB蛋白的表达,且显著抑制白细胞介素1β的生成(P<0.05)。结论普罗布考抗动脉粥样硬化除了降脂作用外,抑制外周血单核细胞Toll样受体4/核因子κB信号转导通路的激活,也可能是其抗炎、抗动脉粥样硬化的重要机制之一,提示普罗布考对防治2型糖尿病并发动脉粥样硬化这类患者有一定的作用。
Aim To study the influence of probucol on the Toll-like receptor 4（TLR4）/nuclear factor-κB（NF-κB） signaling pathway of peripheral blood mononuclear cells in type 2 diabetic mellitus patients with coronary heart disease,so as to further investigate the anti-atherosclerosis mechanism of probucol.Methods 85 patients with type 2 diabetes and carotid atherosclerosis were divided into two groups： probucol treatment group（n=43）,and control group（n=42）.The treatment group were treated with probucol 0.375 g,2 times a day for 24 weeks.The effect of probucol on TLR4,NF-κB expression of peripheral blood monouclear cells was detected by Real-time PCR and Western blotting analysis before and after probucol treatment.The expression of interleukin-1β（IL-β） was detected by ELISA.Results Probucol obviously decreased levels of total cholesterol（TC）,triglyceride（TG）,low density lipoprotein cholesterol（LDLC） and oxidized low density lipoprotein（ox-LDL） in plasma of type 2 diabetes.The expressions of TLR4,NF-κB and IL-β of peripheral blood mononuclear cells were significantly lower in probucol treatment group than that in control（P0.05）.Conclusions Probucol can not only regulate serum lipids,but also has the action of anti-atherosclerosis through inhibiting TLR4/NF-κB expression,which indicate that probucol might have the preventive and treatment role in patients with type 2 diabetes and atherosclerosis.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2009年第12期1009-1012,共4页
Chinese Journal of Arteriosclerosis
