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固始-安卡鸡F_2资源群△-9脂肪酸脱氢酶基因第4、5、6外显子SNP及其与脂肪酸相关性研究

Study on SNP of △-9 Desaturase Gene Extron 4,5,6 and Its Correlation with Some Fatty Acids in F_2 Chickens Derived from Gushi-Anka Chick
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摘要 利用PCR-SSCP(single strand conformation polymorphism)方法,对固始-安卡鸡F2代资源群△-9脂肪酸脱氢酶基因第4、5、6对外显子多态性进行分析,发现存在一个单核苷酸多态(SNP,single nucleotide polymorphisms)位点。在△-9脂肪酸脱氢酶基因第4外显子编码区(89nt)发生了碱基的转换突变(G→A)。在整个F2代资源群中进行了该多态位点与脂肪酸性状的相关分析,结果表明:在8个脂肪酸性状中,AA型个体十六碳二烯酸(C16∶2)含量显著高于GG型和GA型个体(P<0.05);家系1中AA型的C16∶0、C16∶2、C18∶0含量均显著高于GG型和GA型(P<0.05);家系3中AA型的C14∶0含量显著高于GG型和GA型(P<0.05)。 PCR - SSCP technology was used to analyze the polymorphisms of extron 4, 5, 6 of △- 9 desaturase gene in F2 chick- ens derived from Gushi chick to Anka chick. A SNP locus and G--A mutation at 89 nt of the extron 4 were found. The correlation statistical analysis indicated that F2 chickens with genotype AA had significantly higher C16:2 content (P 〈 0. 05 ). Family 1 chickens with genotype AA had significantly higher C16: 0, C16: 2, C18:0 content ( P 〈 0. 05 ). Family 3 chickens with genotype AA had signif- icantly higher C14:0 content (P 〈0.05).
出处 《江西农业学报》 CAS 2010年第1期143-147,共5页 Acta Agriculturae Jiangxi
基金 国家科技支撑计划(2008BADB2B07-5) 国家公益性行业(农业)科研专项(nyhyzx07-039) 国家蛋鸡产业技术体系项目资助 国家农业科技成果转化资金项目(2009D00103) 河南省杰出人才创新基金(074200510009)
关键词 Δ-9脂肪酸脱氢酶基因 单核苷酸多态性 PCR-SSCP 脂肪酸 Chicken △- 9 desaturase gene SNP PCR - SSCP Fatty acid
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