摘要
目的:采用射频低温等离子体,研究庚胺在不同模式下对纯钛表面改性后的理化特征及对不同细胞粘附的影响。方法:利用能谱仪表征不同处理表面的结构、化学成分。同时通过体外培养、细胞计数和荧光显微镜观察研究两种细胞在不同表面的粘附情况。结果:连续波偶连脉冲模式表面引入的氨基最多,成骨细胞在此组表面的粘附显著优于其他组,成纤维细胞则相反,在氨基改性表面早期的粘附明显少于未处理组。结论:等离子体活化的纯钛能影响不同细胞的生长,加速成骨细胞粘附及生长的同时,抑制成纤维细胞的早期贴附。
Objective： Heptylamine was plasma polymerized onto titanium implant using a RF plasma source under lower temperature operating at different process parameters. The effects of modification on the chemical properties of titanium and on the adhesion of different cells were evaluated. Methods： The elemental composition and amino-- group concentration were measured with X--ray photoelectron spectroscopy. In parallel, in vitro evaluation of adhe- sion of osteoblast and fibroblast on samples was carried out by cell counting and fluorescence microscope observa tion. Results： The amino- group concentration on plasma modified surface under continuous wave- combined pulsed wave mode was the highest, with the best adhesion of osteoblast at initial stage. On the contrary, the count of fibroblast on untreated Ti was found to be the most. Conclusion： The amino--group plasma modified Ti surface can affect the growth of different ceils on biomaterials, accelerate the adhesion and growth of Osteoblast around im- plant, and inhibit the initial adhesion of fibroblast at the same time.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2010年第1期11-14,18,共5页
Journal of Oral Science Research
基金
中国教育部公派留学项目资助(编号:2007104445)
中央高校基本科研业务费专项资金资助(编号:421030130431)
关键词
牙种植体
钛
低温等离子体氨基
Dental implants Titanium Lower temperature plasma Amino--group
