摘要
以刺五加不定芽为外植体,采用二次回归正交旋转设计方法,对刺五加初代培养中6-BA和NAA的浓度配比进行筛选。通过对所建模型的分析得出:6-BA的最优浓度为0.72～1.28mg/L,NAA的最优浓度为0.05～0.15mg/L,其可信度达95%。
Quadratic regressive orthogonal revolving design experiment was used to select optimum concentration of 6-BA and NAA in WPM medium for shoot propagation of Acanthopanax senticosus.The result showed that the most of shoots achieved by the 6-BA and NAA concentrations were in a rang of 0.72-1.28 mg/L and 0.05-0.15 mg/L respectively.The probability of the event was 95%.
出处
《中国农学通报》
CSCD
北大核心
2010年第4期85-88,共4页
Chinese Agricultural Science Bulletin
基金
辽宁省"十一五"重大项目"设施农业高效生产技术集成与示范"(2006215001)资助
关键词
二次正交旋转设计
刺五加
组织培养
quadratic orthogonal revolving experiment Acanthopanax senticosus tissue culture
