摘要
目的运用细胞遗传学和分子遗传学方法,对本院258例慢性粒细胞性白血病(CML)患者进行分析,探讨CML中Ph染色体的有关特点及临床意义。方法采用骨髓直接法和短期培养法制备染色体,应用R显带技术对258例CML患者进行核型分析;对其中22例患者运用双色双融合探针(DC-DF-bcr/abl)进行FISH检测Ph易位信号特征。结果11例(4.26%)为Ph(-);247例(95.74%)为Ph(+),其中15例(6.07%)为Ph染色体变异易位。结论染色体核型分析有助于慢粒的诊断和鉴别诊断、判断疗效;FISH检测能给予精确的分子诊断。CML变异性Ph易位广泛累及除9,22外多条染色体。
Objective To explore the characterization and import or Ph chromosome,we analysis 258 cases with chromic myelogenous leukemia using cytogentics and molecular genetics. Methods Chromosome preparation was made using direct method or short-term culture of bone marrow cells. Karyotypic analysis for all 258 cases was carried out by R-banding technique;To detect Ph chromosome translocation in molecular level, metaphase fluorescence in situ hybridization （ FISH ） assays were performed for 22 eases among them using DC-DF-ber/abl probe. Results 11 cases（4.26% ） were Ph（-） ;247 cases（ 95.74% ） were Ph （ ＋ ）, among them 15 cases （6.07%） were variant Ph chromosome translocation. Conclusion Karyotypic analysis is useful for diagnosing, differential diagnosis and detecting curative effect ; FISH provides accurate molecular diagnosis for CML. variant Ph-chromosome translocations in CML involved extensive chromosomes in a varying frequencies and ways except chromosomes 9 and 22.
出处
《四川医学》
CAS
2010年第2期177-179,共3页
Sichuan Medical Journal
关键词
慢性粒细胞性白血病
PH染色体
细胞遗传学
FISH
变异易位
chromic myelogenous leukemia （CML）
Ph chromosome
cytogentics
fluorescence in situ hybridization （FISH）
variant translocation
