摘要
本研究探讨135例非单纯Ph染色体慢性髓系白血病(CML)的染色体检查结果并进行细胞遗传学分析。135例CML染色体均采用骨髓细胞直接法和/或短期培养法制备,应用R显带技术对其进行显带分析。结果表明:经染色体检测的1 210例的CML中135例有非单纯Ph染色体异常。本组135例非单纯Ph染色体CML中,慢性期87例、加速期21例、急变期27例。87例慢性期患者中,14例伴简单变异易位,22例伴复杂变异易位,其余的伴其他染色体异常,其中伴8号染色体三体4例,伴双Ph4例,伴i(17)5例;在21例加速期患者中,伴8号染色体三体4例,伴双Ph4例,伴i(17)3例;在27例急变期患者中,2例伴简单变异易位,3例伴复杂变异易位,其余的伴其他染色体异常,其中伴8号染色体三体5例,伴双Ph5例,伴i(17)2例。本组常见额外染色体异常检出率高低依次为+Ph、+8、i(17)、-Y、+19和+21。本组有16例伴简单变异易位,25例伴复杂变异易位。结论:CML是起源于多能干细胞的恶性血液病。染色体核型分析对CML的诊断、预后、发病机制的探讨和治疗方案的选择都具有重要的价值。
The purpose of this study was to investigate 135 cases of chronic myelogenous leukemia with non-simple Philadelphia chromosome and to analyze their cytogenetic date. Chromosome preparations in 135 cases of patients were performed by using direct method and/or short-term culture, and karyotyping was performed with R-banding technique. The results showed that the overall frequency of chronic myelogenous leukemia with non-simple Philadelphia chromosome （based on 1210 cases of chromosome detection in chronic myelogenous leukemia） was 11. 16%, which included 87 cases of chronic phase, 21 cases of accelerated phase and 27 cases of blastic phase. Among 87 cases of patients in chronic phase, 14 cases were with simple variant translocation and 22 cases had complex variant translocation while the others were with other chromosomal abnormalities including 4 cases of ＋ 8,4 cases of ＋ Ph and 2 cases of i （ 17 ） ; among 21 cases of patients in accelerated phage, 4 cases were with ＋ 8 and 4 cases were with ＋ Ph while 3 cases were with i（ 17 ） ; among 27 cases of patients in blastic phage, 2 cases were with simple variant translocation and 3 cases had complex variant translocation while the others were with other chromosomal abnormalities including 5 cases of ＋ 8, 5 cases of ＋ Ph and 2 cases of i（ 17 ）. The detection rate of extra chromosomal abnormalities in this group of 135 cases patient were ＋ Ph, ＋ 8, i（ 17 ）, -Y, ＋ 19 and ＋ 21 in order. There were 16 cases with simple variant translocation and 25 cases with complex variant translocation in in this group of 135 cases. It is concluded that karyotype analysis is helpful in diagnosis, prognosis, pathogenesis and treatment selection for chronic myelogenons leukemia
出处
《中国实验血液学杂志》
CAS
CSCD
2008年第5期997-1001,共5页
Journal of Experimental Hematology
