摘要
目的:探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)KAI1基因表达下调和淋巴管生成对淋巴结转移是否具有协同作用。方法:应用兔抗人多克隆抗体KAI1和兔抗人抗Podoplanin单克隆抗体(EliVision法)对68例NSCLC组织石蜡切片进行染色,Logistic回归分析判断转移抑制基因KAI1的表达和淋巴管生成与淋巴结转移的关系。结果:NSCLC淋巴结阳性组和淋巴结阴性组KAI1表达阳性率分别为30.3%和88.6%,两者差异有统计学意义(P<0.01)。肿瘤间质中淋巴结阳性患者淋巴管密度(lymphatic vessel density,LVD)为15.73±0.74,明显高于淋巴结阴性患者8.31±0.32(P<0.01)。Logistic回归分析显示,仅LVD对淋巴结转移有影响(P<0.05),其相对危险度(OR)为2.065,95%可信区间(1.202,3.547);而KAI1表达下调无统计学意义(P=0.375),95%可信区间为(0.003,8.714)。NSCLC患者转移抑制基因KAI1的表达下调和肿瘤间质中淋巴管生成对淋巴结转移无明显协同作用(P>0.05)。结论:肿瘤转移抑制基因KAI1表达下调与淋巴管生成对NSCLC淋巴结转移有促进作用,但未见明显协同作用。
Objective：To study the synergy between down-regulation or lack of KAI1 （one of metastasis suppressor genes） and metastasis of lymph node in non-small cell lung eareinoma（NSCLC）. Methods：The expression of KAI1 and Podoplanin which was used to show lymphatic vessel density （LVD） were detected in 68 paraffin sections of NSCLC with immunohistochemistry （EliVision methods） ,and logistic regression analysis was used to show the relationship between the expression anti metastasis of lymph nodes. Results：The positive rates of KAI1 in group one （ with metastasis of lymph node） and group two （ without metastasis of lymph node） were 30.3 % and 88.6% （ P 〈 0.01 ） , respectively. The difference of LVD was significant between group one （ 15.73 ± 0.74） and group two （ 8.31 ± 0.32） （ P 〈 0.01 ）. Logistic regression analysis showed that LVD had an impact on metastasis of lymph node（ P 〈 0.05 ） ; the odds risk（OR） was 2. 065 and the confidence interval of 95% was （ 1. 202,3. 547 ）. The down-regulation of KAI1 had no statistical significance（ P = 0. 375 ） , and the confidence interval of 95% was（0. 003,8. 714）. There was no significant synergy between LVD and the expression of KAI1 in metastasis of lymph node （P 〉 0.05 ）. Conclusions： There exists no obvious synergies between downregulation of KAI1 and lymphangiogenesis in metastasis of lymph node in non-small cell lung earcinoma.
出处
《蚌埠医学院学报》
CAS
2010年第2期124-126,共3页
Journal of Bengbu Medical College
基金
安徽省教育厅自然科学研究资助项目(KJ2008B53ZC)
关键词
癌
非小细胞肺
肿瘤转移抑制基因
KAI1
淋巴管生成
淋巴管密度
carcinoma,non-small cell lung cancer
tumor metastasis suppressor gene
KAI1
lymphangiogenesis
lymphatie vessel density
