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高表达GRIM-19诱导结肠癌SW480细胞凋亡 被引量：1

Grim-19 overepression induces apoptosis of colon cancer SW480 cells
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摘要 目的:研究干扰素/维甲酸诱导死亡基因(retinoid-interferon-induced mortality,GRIM-19)对结肠癌SW480细胞凋亡的影响。方法:构建GRIM-19真核表达载体(pCMV-Flag-GRIM-19),转染入SW480细胞中,Western blotting检测GRIM-19及凋亡相关蛋白Bal-xl的表达,采用Annexin-V/PI和线粒体膜电位JC-1染色检测SW480细胞的凋亡。结果:成功构建pCMV-Flag-GRIM-19真核表达载体。pCMV-Flag-GRIM-19质粒转染SW480细胞后,GRIM-19的表达上调,凋亡抑制蛋白Bcl-xl的表达则下调。转染空质粒pCMV-Flag组SW480细胞凋亡率为(7.7±1.39)%,转染pCMV-Flag-GRIM-19质粒后SW480细胞凋亡率为(15.0±2.52)%(P<0.05)。线粒体膜电位检测显示转染空质粒pCMV-Flag组SW480细胞膜电位降低细胞为(7.5±2.09)%,而转染pCMV-Flag-GRIM-19后细胞线粒体膜电位降低细胞为(17.5±3.07)%(P<0.05)。结论:GRIM-19体外转染可有效诱导结肠癌SW480细胞凋亡。 Objective：To investigate the effect of retinoid-interferon-induced mortality （GRIM-19） gene on the apoptosis of colon cancer.Methods：A GRIM-19 eukaryotic expression vector （pCMV-Flag-GRIM-19） was constructed and transfected into SW480 cells.Expressions of GRIM-19 and apoptosis-related proteins were detected by Western blotting analysis.Apoptosis of SW480 cells was measured by Annexin-V/PI assay and mitochondrial membrane potential JC-1 staining.Results：The GRIM-19 eukaryotic expression vector pCMV-Flag-GRIM-19 was successfully constructed.Expression of GRIM-19 in SW480 cells was up-regulated and that of apoptosis-related protein Bcl-xl was down-regulated after transfection with pCMV-Flag-GRIM-19.Apoptosis rate was （7.7±1.39）% in SW480 cells transfected with pCMV-Flag empty vector and （15.0 ± 2.52）% in pCMV-Flag-GRIM-19 transfected cells （P〈0.05）.Mitochondrial membrane potential was decreased in （7.5±2.09）% of pCMV-Flag transfected cells and （17.5±3.07）% of pCMV-Flag-GRIM-19 transfected cells （P〈0.05）.Conclusion：In vitro GRIM-19 transfection can effectively induce apoptosis of colon cancer SW480 cells.
作者 王桂华 罗学来 孙黎 邓豫 王珅 李兆明 李小兰 陶德定 胡俊波 龚建平
机构地区 华中科技大学同济医学院附属同济医院胃肠外科中心
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2010年第1期67-70,共4页 Chinese Journal of Cancer Biotherapy
基金 国家自然科学基金资助项目(No. 30471673 30872472 30800569) 国家重点基础研究发展计划( 973 )项目( No.2009CB521802)~~
关键词 结肠肿瘤 GRIM-19 凋亡 Annexin-V/PI 线粒体膜电位 colon cancer GRIM-19 apoptosis Annexin-V/PI mitochondrial membrane potential
分类号 R735.35 [医药卫生—肿瘤] R730.54 [医药卫生—肿瘤]
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参考文献20

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中国肿瘤生物治疗杂志
2010年 第1期

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