摘要
[目的]研究制备壳寡糖的方法。[方法]运用基因工程的方法,将人工构建的含有壳聚糖水解酶基因的质粒载体转入大肠杆菌体内并诱导其表达。[结果]可溶的活性壳聚糖酶在大肠杆菌中的表达量高于300mg/L。[结论]该重组壳聚糖酶与天然来源的壳聚糖酶具有完全相同的比活力,能专一性切断β-1,4-糖苷键,将壳聚糖降解为不含单糖的壳寡糖。
[Objective]The research aimed to study the preparation of chito-oligosacchaside.[Method]Gene engineering was used to transfer plasmid,which contained the gene of chitosan-hydrolyase,into E.coli.Then the enzyme was induced to express.After purification,chitosanhydrolyase was used to degrade chitosan.[Result]The amount of the expression of chitosan-hydrolyase in E.coli was higher than 300 mg/L.[Conclusion]The recombinate chitosan-hydrolyase had the same specific activity with the chitosan-hydrolyase from nature.It could specifically break β-1,4 glycosidic bonds and degrade chitosan to chitosan-hydrolyase with no monosaccharide.
出处
《安徽农业科学》
CAS
北大核心
2010年第4期1684-1686,共3页
Journal of Anhui Agricultural Sciences
基金
2008年上海市大学生创新性试验计划项目
2008年同济大学"大学生创新性实验计划资助项目"
关键词
壳聚糖
壳寡糖
壳聚糖水解酶
Hitosan
Chito-oligosacchaside
Chitosan-hydrolyase
