摘要
为评价野生稻与亚洲栽培稻的遗传多样性及其变异关系,21对SSR引物被用于研究广泛地理分布的17份野生稻(其中11份O.rufipogon和6份O.nivara)和22份亚洲栽培稻(13份indica和9份japonica)样本。271条多态性片段被扩增出,占总扩增片段的96.10%。野生稻多态性片段的百分比(平均达64.30%)及Nei’s遗传多样性值(GD)明显高于亚洲栽培稻,表明野生稻比亚洲栽培稻具更丰富的遗传多样性。UPGMA聚类分析显示野生稻的两个类群(O.rufipogon和O.nivara)关系密切,但在遗传上存在明显的分化,支持其作为两个独立物种的分类观点。野生稻中籼粳分化不很明显,亚洲栽培稻的籼粳亚种分化明显。亚洲栽培稻与多年生普通野生稻(O.rufipogon)关系更为密切,符合异源起源的遗传分化模式。
To explore the genetic diversity and variation of common wild rice and Asian cultivated rice, 17 accessions of common wild rice （11 O. rufipogon and 60. nivara） and 22 cultivars of Asian cultivated rice （13 indica and 9 japonica） collected worldwide were analyzed by 21 ISSR primers. The polymorphic fragments percentage was 96.10% and 271 polymorphic fragments were detected. A comparison of the polymorphic fragments percentage and Nei＇s genetic diversity （GD） indicated that the genetic diversity of common wild rice was obviously higher than that of Asian cultivated rice. The UPGMA cluster analysis revealed that there was a close relationship between O. rufipogon and O. nivara, but dis.tinct genetic differentiation was detected between them, which supported the point that they were two independent species. The results indicated that the indica-japonica differentiation was not very apparent in many wild rice whereas it was obvious in Asian cultivated rice. According to the cluster analysis, there was a closer consanguineous relationship between Asian cultivated rice and O. rufipogon, which supported the diphyletic origin hypotheses.
出处
《井冈山大学学报（自然科学版）》
2010年第1期118-124,共7页
Journal of Jinggangshan University (Natural Science)
基金
江西省科技计划指导性项目(2006021)
关键词
ISSR
亚洲栽培稻
野生稻
遗传多样性
ISSR fingerprints
Asian cultivated rice
wild rice
genetic diversity
