摘要
利用醇沉-萃取法,从生物转化发酵液中提取(2S,5S)-2,5-己二醇,并考察醇沉、萃取和结晶的工艺条件.结果表明,醇沉时,加入的乙醇与浓缩发酵液的最佳体积比为2∶1,pH值对醇沉效果影响显著,强碱和强酸条件下可使大部分杂质析出.经乙酸乙酯连续5次萃取,醇沉浓缩液中2,5-己二醇的萃取率达75.0%.通过优化后的工艺进行分离纯化,发酵液中2,5-己二醇总提取率为49.2%,纯度≥98.0%,(2S,5S)-2,5-己二醇的光学纯度值在提纯前后均为94.5%.
The technological conditions of extracting （2S, 5S）-2,5-hexanediol from fermentation broth by a combination of alcohol precipitation and extraction was investigated. The refined product 2,5-hexanediol was obtained from fermentation broth by centrifugation, alcohol precipitation, extraction, crystallization and recrystallization. The results showed the optimal volume ratio of alcohol to condensed fermentation broth in alcohol precipitation was 2 ： 1. The pH value of broth had great effect on precipitation, most impuritis could be precipitated under strong acidity or basicity condition. And, after five consecutive extractions from concentrated liquid with ethyl acetate, the yield of 2,5-hexanediol could reach 75.0%. By using the optimal process of separation and purification from fermentation broth, the yield of 2,5-hexanediol was 49. 2%, and its purity could be up to 98. 0%. The optical purity value of the refined product （2S, 5S）-2, 5-hexanediol could be kept at 94. 5% after purification process.
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2010年第2期196-199,共4页
Journal of Huaqiao University(Natural Science)
基金
福建省泉州市科技计划重点项目(2006G05)
关键词
(2S
5S)-2
5-己二醇
醇沉
萃取
发酵液
（2S,5S）-2,5-hexanediol
alcohol precipitation
extraction
fermentation broth
