摘要
目的探讨Ｅ－选择素对局灶缺血性脑皮质血流的影响。方法用Ｅ－ｓｅｌｅｃｔｉｎＬｅｃｔｉｎＤｏ－ｍａｉｎ（以下Ｅ－选择素）Ｎ－末端２３－３０氨基酸残基合成的寡肽（Ｏｌｉｇｏｐｅｐｔｉｄｅ）１０ｍｇ／ｋｇ，或伪Ｅ－ｓｅｌｅｃｔｉｎ或等量生理盐水，在ＳＨＲ（自发高血压性大鼠）ＭＣＡ／ＣＣＡ（大脑中动脉／颈总动脉）闭塞２ｈ后ＣＣＡ再灌注的模型中，静脉内缓慢注入，用激光多普勒血流测定仪从ＳＨＲ缺血前１０ｍｉｎ起至ＭＣＡ／ＣＣＡ闭塞２ｈ、ＣＣＡ再灌注后３０ｍｉｎ止，在大脑皮质背侧测定脑血流的变化。结果生理盐水组、伪Ｅ－选择素和Ｅ－选择素组的局部脑血流分别是２３％、２９％和５４％，有统计学意义（Ｐ＜０．０５）。
Objective To research the effect of E selectin on cerebral blood flow in focal ischemic cortex Methods Asynthetic oligopeptide(amino acid residues 23 30 from N terminal) of E selectin lectin domain was dissolved in physiological saline and injected intravenously at a dosage of 10mg/kg、2mg/kg、E selectin or scrambled E selectin,or salin before SHR(spontaneously hypertensive rat)left (middle cerebral artery) and CCA(common carotid artery)were occlused The CCA was reperfused after 2 hours of MCA/CCA occlusin Regional cerebral blood flow (rCBF) was moritored with laser Doppler flowmetry Results rCBF was 54%、29%、and 23% respectively in E selectin 10mg/kg、scrambled E selectin and saline groups p<0 05,student ,s test Conclusion E selectin can effectively increase rCBF of cortex after SHR ischemia and reperfusion
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
1998年第6期333-335,共3页
Journal of Apoplexy and Nervous Diseases
关键词
E-选择素
脑缺血
血流动力学
E selectin Cerebral ischemia Laser Doppler flowmetry Treatment
