摘要
目的建立测定豆粕及发酵豆粕中大豆异黄酮含量的毛细管电泳方法。方法采用胶柬毛细管电泳法（MECC），未涂层弹性石英毛细管（50μm×60cm，50cm）；压力进样，压力：3．0×10^3Pa；进样时间：5。；分离电压：17kV；柱温：20℃；检测波长：254nm；运行缓冲液：15mmol·L^-1十二烷基硫酸钠、5％甲醇的10mmol·L^-1硼砂溶液（pH9．4）。结果测得6种大豆异黄酮组分在24min内能完全分离并具有良好的线性关系；平均加样回收率（n=5）在99．6％～103．5％之间，RSD为1．8％～2．3％。结论方法准确、可靠、经济、操作简便，适合于豆粕及发酵豆粕中大豆异黄酮含量的测定。
OBJECTIVE To establish a mieellar electrokinetic capillary chromatography （MECC） method for the determination of soybean isoflavones in soybean meal and fermented soybean meal. METHODS The separation was performed on a fused and uneoated silie capillary of 50 μm × 60 cm （50 cm of effective length）. 10 mmol · L-1 sodium borate （ pH 9.4 ） containing 15 mmol· L^ -1 sodium dodeeyl sulfate （SDS） and 5% methanol was used as running buffer. Samples were injected hydrodynamically （5 s, 3.0 × 10^3Pa） into the capillary. The applied voltage was 17 kV and the wavelength of detection was set at 254 nm. The temperature was at 20℃. RESULTS The six soybean isoflavones were completely isolated and showed good linear relationships. The average recoveries （n = 5 ） were 99. 6% - 103.5% and RSDs were 1.8% -2.3% for daidzin, daidzein, genistin and genistein. CONCLUSION This method is accurate, reliable, economic, easy to operate and suitable for the determination of soybean isoflavones in soybean meal and fermented soybean meal.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2010年第3期223-227,共5页
Chinese Pharmaceutical Journal
基金
福建省自然科学基金资助项目(2009J01031)
国务院侨办科研基金资助项目(09QZR06)
华侨大学科研基金资助项目(09HZR08)
关键词
豆粕
发酵豆粕
胶束毛细管电泳
大豆异黄酮
soybean meal
fermented soybean meal
mieella electrokinetie capillary chromatography
soybean isoflavone
