摘要
本文利用合成的降钙素基因相关肽的抗原决定簇Ｔｙｒ－ＣＧＲＰ（２７～３７）制备抗ＣＧＲＰ抗血清，用Ｉｏｄｏｇｅｎ做氧化标记抗原，以免疫沉淀剂行Ｂ与Ｆ分离，从而建立了降钙素基因相关肽的放免测定方法，方法灵敏度为６ｐｇ／ｍｌ回收率６５％～８０％，批内ＣＶ７．７６％，批间ＣＶ１１．３８％，正常人血浆ＣＧＲＰ水平４３．５７±５．２８ｐｇ／ｍｌ，急性心梗塞患者血浆ＣＧＲＰ水平７０．２４±６．２３ｐｇ／ｍｇ。
Synthetic antigenin diterminant of Calcitonin Gene Related Peptide (CGRP), tyr-CGRP(27~37), were used to preparate anti-CGRP antiserum. As oxidizer, Iodogen was natural-ized in making labeled antigen. Immuno-pricipititing reagent was adopted for separating bindingphase from free phase. Then, the radioimmunoassay of CGRP was have been created. The sensi-tivity was 6 pg/ml. The recovery ratio was 65%~80%. The intra-and inter-assay coeficient ofvariation (CV) were 7. 76% and 11. 38%. The CGRP level in serum of healthe human group andacute myocardial infarction patient were 43. 57±5. 28 pg/ml and 70. 24±6. 23 pg/ml. There wassignificant diffrence (P < 0.05) between the two group. In cardiopulmonary bypass (CBP)pateint's serum, three was diffrence about CGRP level beteen operation stage and before operationstage. (P<0. 05) In 24 houre after operation, serum CGRP level was significant higher than bothoperation stages' and before operation stages' (P<0.05).
出处
《海军总医院学报》
1998年第4期211-213,共3页
Journal of Naval General Hospital of PLA
关键词
降钙素
基因相关肽
放射免疫
急性
心肌梗塞
Calcitonin gene related peptide (CGRP) Radioimmunoassay Acute my-ocardial infarction Cardiopulmonary bypass (CPB)
