摘要
为研究鼬科动物犬瘟热病毒(canine distemper virus,CDV)毒株的变异特性,对分离自不同地区貉、貂的5个CDV毒株和1个CDV国内弱毒疫苗株(vacChina)的H基因进行了核苷酸测序,并与12个参考毒株构建系统发生树。结果表明:5个流行毒株之间H基因核苷酸序列同源性均达到97.5%以上,vacChina与5个流行毒株的H基因核苷酸序列同源性普遍较低(90.5%～91.8%),而与国外疫苗株Convac和Onderstepoort的H基因核苷酸同源性达96.8%和97.2%。流行毒株之间H蛋白氨基酸同源性差别不大(97.1%～100.0%),流行毒株与vacChina疫苗株H蛋白氨基酸同源性普遍较低(89.7%～91.8%),vacChina与Onderstepoort、Convac有着很高的同源性,其H蛋白氨基酸同源性分别为97.1%、95.6%。结果分析显示,CDV的流行和免疫失败现象的发生与国内所用疫苗毒株和野毒株的遗传关系甚远有关。
To characterize the variability of recent field isolates of 5 canine distemper virus （CDV） strains from different hosts and geographical areas, we conducted nueleotide sequence analysis of the H gene encoding the haemagglutinin protein. Pronounced differences between 5 field isolates were revealed in comparison to the attenuated vaccine strains vacChina, Convac and Onderstepoort. Phylogenetic tree was constructed with H gene of 5 CDV isolates, vaccine strain vaeChina and 12 CDV strains in GeneBank. Results： The homology of nucleic acid sequences among the 5 isolates was high （97.6%--99.9%）. The homology of nucleic acid sequences between vacChina and 5 isolates was low（90.5%--91.8% ）while high with foreign vaccines, vacConvac（ H：96.8% ） and vacOnderstepoort（H：97.2% ）. The difference of amino acid homology of H protein among 5 isolates was not high（97.1%--100.0% ）. The homology of H protein between vacChina and 5-isolates was low,with H protein 89.7%--91.8%. VacChina and Onderstepoort, Convac had high homology of amino acid in H protein. Conclusion： The CD prevalence in several provinces of China this summer may be due to the far heredity between vaccine strain and field strains.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2010年第1期75-80,共6页
Journal of Jilin Agricultural University
基金
青岛市科技发展计划项目(O5-2-JC-78)
关键词
犬瘟热病毒
克隆
遗传分析
同源性比较
canine distemper virus
cloning
hereditary analysis
homology comparison
