摘要
目的:观察重组腺相关病毒(rAAV)介导的人骨形态发生蛋白2(hBMP2)对大鼠实验性牙槽骨缺损的修复作用。方法:将30只实验大白鼠随机分为实验组和对照组。在两组动物下颌1 1牙间牙槽骨人工制备2mm×2mm骨缺损区。实验组骨缺损区植入浸有携目的基因hBMP2和标记基因绿色荧光蛋白(GFP)的重组腺相关病毒液(rAAV-hBMP2-GFP)的明胶海绵块,对照组植入只浸有携GFP的空病毒液(AAV-GFP)的明胶海绵块。术后15d、30d、60d分别处死动物,进行放射学和组织学观察。结果:1两组样本牙槽骨缺损处的骨密度值随时间的增加而增高;在15d、30d、60d,实验组样本牙槽骨缺损处骨密度值(0.062,0.236,0.456)均高于对照组(0.054,0.158,0.216),其中30d、60d两组有显著性差异(P<0.05,P<0.01)。2术后15d两组样本牙槽骨缺损处周边组织均可见到GFP;术后30d、60d,实验组样本较对照组绿色荧光强度明显加强。3实验组:术后15d,牙槽骨人工缺损区周围成纤维细胞和毛细血管生长活跃,周边有骨样组织和少量的骨组织形成。术后30d,骨缺损处新骨数量增多。术后60d,缺损区几乎被新生骨组织充满;对照组:术后15d可见少量炎性细胞,成纤维细胞和毛细血管生长活跃;术后30d,骨缺损区周边可见成骨细胞和少量骨样组织;术后60d,骨缺损区边缘有少量新骨沉积,腔内被纤维组织充满。结论:rAAV-hBMP2-GFP成功修复大鼠实验性牙槽骨缺损。
Objective： To study the treatment of experimental alveolar bone defect with BMP-2. Methods：Alveolar bone defect was made in 30 rats at 1 1 region. In fifteen rats（experimental group）, the defect was filled with gelatin sponge infiltrated by rAAV hBMP2- GFP solution; In fifteen rats （control group）, the defect was filled with gelatin sponge infiltrated by AAV- GFP solution . The peripheral tissue coating alveolar bone defect was harvested for radiological and histopathologic study after 15 days, 30 days and 60 days. Results：（1）The density of alveolar bone defect measured by computer increased with time in two groups. The density of sample （30days, 60days） in experimental group was obviously higher than that in control group（P〈0. 05,P〈0. 01）. （2）The green fluorescent protein（GFP） in all samples was found after 15 days. The intensity of GFP in tissue around alveolar bone defect （30days, 60days） in experimental group was higher than that in control group. （3） The main histopathologic changes of experimental group： fibroblast and capillary tachyauxesis, osteoid tissue and small amount neoformative bone tissue appearance （15 days）; neoformative bone tissue enlarging （30 days）; neoformative bone tissue maturity （60 days）. Control group： fibroblast and capillary tachyauxesis（15 days） ;osteoblast appearance around defect（30 days） ;small amount neoformative bone tissue around defect （60 days）. Conclusion： rAAV hBMP2- GFP succeed in renovation on rat experimental alveolar bone defect.
出处
《陕西医学杂志》
CAS
2010年第2期131-133,139,共4页
Shaanxi Medical Journal
基金
陕西省卫生厅科学研究基金(06D40)
陕西省科技计划项目(2006K14-G3)
西安交通大学口腔医院青年基金(0603)
