摘要
目的:了解长春地区部分医院超广谱β-内酰胺酶(ESBLs)基因在革兰阴性杆菌中的分布及类型,为合理使用抗生素提供依据。方法:对165株临床分离革兰阴性杆菌采用PCR方法分别扩增SHV、TEM和CTX基因片段。PCR产物经测序后,登录GenBank查询并比较基因类型。结果:在165株革兰阴性杆菌中检出耐药基因及多重耐药基因68株,其中TEM型检出率最高(12.12%),其余依次为CTX-M-1型(4.24%),SHV型(3.64%)和CTX-M-9型(3.03%);同时检出SHV+TEM型、TEM+CTX-M-1型、TEM+CTX-M-9型、TEM+CTX-M-1+CTX-M-9型和SHV+TEM+CTX-M-1+CTX-M-9型等多组多重耐药基因。随机选择扩增阳性的PCR产物进行测序,得到SHV-11亚型、TEM-1亚型、CTX-M-3亚型、CTX-M-15亚型、CTX-M-14和CTX-M-18亚型。结论:长春地区部分医院革兰阴性杆菌ESBLs的基因类型主要为TEM-1亚型、SHV-11亚型、CTX-M-14亚型和CTX-M-18亚型。
Objective To illustrate the genotypes and distribution of the extended-spectrmu 13-1actamases （ESBLs） of gram-negative bacterial isolates from several hospitals in Changchun, Jilin, China and to provide the reliable basis for antibiotic application. Methods Drug-resistant genes associated with ESBLs in gram-negative bacterial isolates were amplified by PCR. PCR products were sequenced and compared with the genotypes reported in GenBank. Results 68 strains of 165 isolates exhibited drug resistance such as TEM-type （12. 12%）, CTX-M-1 type （4.24%）, SHV-type （3.64%） and CTX-M-9 type （3. 03%）. The multi-drug resistant genes including SHV ＋ TEM-type, TEM ＋CTX-M-1 type, TEM ＋ CTX-M-9 type, TEM ＋ CTX-M-1 ＋ CTX-M-9 type and SHV ＋ TEM ＋ CTX-M-1 ＋ CTX-M-9 type were also detected. The positive PCR amplification products were randomly selected and sequenced, the SHV 11 subtype, TEM-1 subtype, CTX-M-3 subtype and the CTX-M-15 subtype, CTX-M-14 and CTX-M-18 subtype were obtained. Conclusion The genotypes of ESBLs in gram-negative bacterial isolates mainly include TEM-1 subtype, CTX-M 14 subtype, CTX-M-18 subtype, SHV-11 in Changchun area.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2010年第1期205-209,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(200705374)
关键词
超广谱Β-内酰胺酶
革兰阴性杆菌
聚合酶链反应
extended-spectrum β-lactamases
gram negative bacilli
polymerase chain reaction
